Team:Paris Liliane Bettencourt/Notebook/2011/10/15/

From 2011.igem.org

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==Kevin==
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===Results of transformation===
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pVeg + SpoVG - YFP:tetR is OK (fluorescence at illuminatool).<br>
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YFP:tetR - Double Term isn't good (after 12 PCR Colony, no one are ok).
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=== Creating pVeg + SpoVG - YFP:tetR - Double Term (the full construct) ===
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Digestion to transformation today.
 +
==Characterization of different strains in order to check if Subtilis or not==
==Characterization of different strains in order to check if Subtilis or not==
===Characterization of ComS===
===Characterization of ComS===

Latest revision as of 20:45, 21 October 2011

Contents

Kevin

Results of transformation

pVeg + SpoVG - YFP:tetR is OK (fluorescence at illuminatool).
YFP:tetR - Double Term isn't good (after 12 PCR Colony, no one are ok).

Creating pVeg + SpoVG - YFP:tetR - Double Term (the full construct)

Digestion to transformation today.

Characterization of different strains in order to check if Subtilis or not

Characterization of ComS

File:ComS exp trans1 16 10.jpg
ComS in exponential phase (trans image)
File:ComS fluo 1 16 10.jpg
ComS in stationnary phase (gfp image)
File:ComS fluo 2 16 10.jpg
ComS in stationnary phase (gfp image)
File:ComS trans 1 16 10.jpg
ComS in stationnary phase (trans image)


File:ComS trans 1 16 10.jpg
ComS in stationnary phase (trans image)
File:ComS trans 2 16 10.jpg
ComS in stationnary phase (trans image)


Characterization of KinA Emittor

File:KinA em exp trans 1 16 10.jpg
KinA EM in exponential phase (trans image)
File:KinA em exp trans 2 16 10.jpg
KinA EM in exponential phase (trans image)
File:KinA em exp trans 3 16 10.jpg
KinA EM in exponential phase (trans image)
File:KinA em trans 1 16 10.jpg
KinA EM in stationnary phase (trans image)


Characterization of KinA Receiver

File:KinA rec exp trans 1 16 10.jpg
KinA REC in exponential phase (trans image)
File:KinA rec exp trans 2 16 10.jpg
KinA REC in exponential phase (trans image)
File:KinA rec trans 1 16 10.jpg
KinA REC in stationnary phase (trans image)


Characterization of RFP

File:Rfp expo fluo 1 16 10.jpg
RFP in exponential phase (rfp image)
File:Rfp expo fluo 2 16 10.jpg
RFP in exponential phase (rfp image)
File:Rfp expo fluo 3 16 10.jpg
RFP in exponential phase (rfp image)


File:Rfp expo trans 1 16 10.jpg
RFP in exponential phase (trans image)
File:Rfp expo trans 2 16 10.jpg
RFP in exponential phase (trans image)
File:Rfp expo trans 3 16 10.jpg
RFP in exponential phase (trans image)


File:Rfp fluo 1 16 10.jpg
RFP in stationnary phase (rfp image)
File:Rfp fluo 2 16 10.jpg
RFP in stationnary phase (rfp image)


File:Rfp trans 1 16 10.jpg
RFP in stationnary phase (trans image)
File:Rfp trans 2 16 10.jpg
RFP in stationnary phase (trans image)



Characterization of T7 autoloop

File:T7 autoloop exp fluo 1 16 10.jpg
KinA REC in exp phase (trans image)
File:T7 autoloop exp fluo 2 16 10.jpg
KinA REC in exp phase (trans image)
File:T7 autoloop exp trans 1 16 10.jpg
KinA REC in stationnary phase (trans image)


File:T7 autoloop exp trans 2 16 10.jpg
KinA REC in exp phase (trans image)
File:T7 autoloop fluo 1 16 10.jpg
KinA REC in exp phase (trans image)
File:T7 autoloop fluo 2 16 10.jpg
KinA REC in stationnary phase (trans image)


File:T7 autoloop trans 1 16 10.jpg
KinA REC in exp phase (trans image)
File:T7 autoloop trans 2 16 10.jpg
KinA REC in exp phase (trans image)


Characterization of TetO

File:Teto exp trans 1 16 10.jpg
KinA REC in exp phase (trans image)
File:Teto exp trans 2 16 10.jpg
KinA REC in exp phase (trans image)
File:Teto exp trans 3 16 10.jpg
KinA REC in stationnary phase (trans image)