Team:Paris Liliane Bettencourt/Notebook/2011/09/04/

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Contents

Kevin

Biobrick the YFP:tetR fusion protein

Let's start again with the good primer to biobrick the protein.

PCR

3 Temperatures of primer annealing: 85 - 58 - 48 °C
Primers : pFX234 FW - pFX234 Reverse CP

Following steps

PCR Purification, digestion in ES, PCR Purification again, Ligation

To be continued

Transformation, sequencing and glycerol.

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