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Team:Paris Liliane Bettencourt/Notebook/2011/09/04/
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Let's start again with the good primer to biobrick the protein. | Let's start again with the good primer to biobrick the protein. | ||
| - | ===PCR=== | + | ====PCR==== |
3 Temperatures of primer annealing: 85 - 58 - 48 °C<br> | 3 Temperatures of primer annealing: 85 - 58 - 48 °C<br> | ||
Primers : pFX234 FW - pFX234 Reverse CP | Primers : pFX234 FW - pFX234 Reverse CP | ||
| - | ===Following steps=== | + | ====Following steps==== |
PCR Purification, digestion in ES, PCR Purification again, Ligation | PCR Purification, digestion in ES, PCR Purification again, Ligation | ||
| - | ===To be continued=== | + | ====To be continued==== |
Transformation, sequencing and glycerol. | Transformation, sequencing and glycerol. | ||
Revision as of 13:59, 4 September 2011
Contents |
Kevin
Biobrick the YFP:tetR fusion protein
Let's start again with the good primer to biobrick the protein.
PCR
3 Temperatures of primer annealing: 85 - 58 - 48 °C
Primers : pFX234 FW - pFX234 Reverse CP
Following steps
PCR Purification, digestion in ES, PCR Purification again, Ligation
To be continued
Transformation, sequencing and glycerol.
