"

From 2011.igem.org

Revision as of 23:07, 3 September 2011 (view source) Danyel.lee90 (Talk | contribs) (→Overview) ← Older edit		Revision as of 23:11, 3 September 2011 (view source) Danyel.lee90 (Talk | contribs) Newer edit →
Line 46:		Line 46:
	=== To Do List ===		=== To Do List ===
		+	in order of priority:
		+
		+
		+	'''Sequencing'''
		+	*S92 = S81 in pHM3
		+
		+	'''Cloning'''
		+	*The plates labelled with orange tape: "Danny's Transfos":

---

Revision as of 23:11, 3 September 2011

Danyel

• Overview
• To do list

Overview

The constructs involved in the tRNA project so far:

• General information : all values are APPROX.
  • tRNA = 100 bp
  • T7 amber = 2.7 kb
  • GFP amber = 720 bp
  • pHyperspank = 101 bp
  • Pveg_spoVG = 120 bp
  • pT7_RBS_GFP_TT = 823 bp

• tRNA amber suppressor
  • tRNA

     S38: in pSB1C3

• tRNA_TT

     S58: in pSB1C3

• Pveg_spoVG_tRNA

     S38 in S24 --- [in progress]

• Pveg_spoVG_tRNA_TT

     S81 :  this contruct in pSB1AK3 (ampR)
     S92 :  this contruct in pHM3 (ampR/tetR) --- sequencing required and transformation in subtilis attempted by Adrien.

• pHyperspank_tRNA

     S38 in S82 --- [in progress]

• pHyperspank_tRNA_TT

     S58 in S82 --- [in progress]

• Proteins with amber mutations
• T7 amber_TT

     S80 : in pSB1AK3

• GFP amber

     [in progress : transformation]

• GFP_TT

     [in progress : transformation]

• other constructs
• pT7_RBS_GFP_TT  :

     S79 :  this construct in ampR plasmid (pSB1AK3?).
     *Functionality has been tested through transformation in BL21 and microscopy. (cf.27/08/11)

To Do List

in order of priority:

Sequencing

• S92 = S81 in pHM3

Cloning

• The plates labelled with orange tape: "Danny's Transfos":