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Revision as of 23:06, 3 September 2011 (view source) Danyel.lee90 (Talk | contribs) (→Overview) ← Older edit		Revision as of 23:06, 3 September 2011 (view source) Danyel.lee90 (Talk | contribs) (→Overview) Newer edit →
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	**Pveg_spoVG = 120 bp		**Pveg_spoVG = 120 bp
	**pT7_RBS_GFP_TT = 823 bp		**pT7_RBS_GFP_TT = 823 bp
		+
	*'''tRNA amber suppressor'''		*'''tRNA amber suppressor'''
	**tRNA		**tRNA

---

Revision as of 23:06, 3 September 2011

Danyel

• Overview
• To do list

Overview

The constructs involved in the tRNA project so far:

• General information : all values are APPROX.
  • tRNA = 100 bp
  • T7 amber = 2.7 kb
  • GFP amber = 720 bp
  • pHyperspank = 101 bp
  • Pveg_spoVG = 120 bp
  • pT7_RBS_GFP_TT = 823 bp

• tRNA amber suppressor
  • tRNA

     S38: in pSB1C3

• tRNA_TT

     S58: in pSB1C3

• Pveg_spoVG_tRNA

     S38 in S24 --- [in progress]

• Pveg_spoVG_tRNA_TT

     S81 :  this contruct in pSB1AK3 (ampR)
     S92 :  this contruct in pHM3 (ampR/tetR) --- sequencing required and transformation in subtilis attempted by Adrien.

• pHyperspank_tRNA

     S38 in S82 --- [in progress]

• pHyperspank_tRNA_TT

     S58 in S82 --- [in progress]

• Proteins with amber mutations
• T7 amber_TT

     S80 : in pSB1AK3

• GFP amber

     [in progress : transformation]

• GFP_TT

     [in progress : transformation]

• Reporting constructs
• pT7_RBS_GFP_TT  :

     S79 :  this construct in ampR plasmid (pSB1AK3?).
     *Functionality has been tested through transformation in BL21 and microscopy. (cf.27/08/11)