Team:Paris Liliane Bettencourt/Notebook/2011/09/04

From 2011.igem.org

(Difference between revisions)

Revision as of 23:06, 3 September 2011

Danyel

Overview
To do list

Overview

The constructs involved in the tRNA project so far:

General information : all values are APPROX.
- tRNA = 100 bp
- T7 amber = 2.7 kb
- GFP amber = 720 bp
- pHyperspank = 101 bp
- Pveg_spoVG = 120 bp
- pT7_RBS_GFP_TT = 823 bp
tRNA amber suppressor
- tRNA

     S38: in pSB1C3

tRNA_TT

     S58: in pSB1C3

Pveg_spoVG_tRNA

     S38 in S24 --- [in progress]

Pveg_spoVG_tRNA_TT

     S81 :  this contruct in pSB1AK3 (ampR)
     S92 :  this contruct in pHM3 (ampR/tetR) --- sequencing required and transformation in subtilis attempted by Adrien.

pHyperspank_tRNA

     S38 in S82 --- [in progress]

pHyperspank_tRNA_TT

     S58 in S82 --- [in progress]

Proteins with amber mutations
T7 amber_TT

     S80 : in pSB1AK3

GFP amber

     [in progress : transformation]

GFP_TT

     [in progress : transformation]

Reporting constructs
pT7_RBS_GFP_TT :

     S79 :  this construct in ampR plasmid (pSB1AK3?).
     *Functionality has been tested through transformation in BL21 and microscopy. (cf.27/08/11)

@@ Line 25: / Line 25: @@
 *pHyperspank_tRNA_TT
        S58 in S82 --- [in progress]
-*pT7_RBS_GFP_TT  :
-      S79 :  this construct in ampR plasmid (pSB1AK3?).
-      *Functionality has been tested through transformation in BL21 and microscopy. (cf.27/08/11)
 *'''Proteins with amber mutations'''
@@ Line 36: / Line 34: @@
 * GFP_TT
        [in progress : transformation]
+*'''Reporting constructs'''
+*pT7_RBS_GFP_TT  :
+      S79 :  this construct in ampR plasmid (pSB1AK3?).
+      *Functionality has been tested through transformation in BL21 and microscopy. (cf.27/08/11)