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Team:Paris Liliane Bettencourt/Notebook/2011/09/04
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Danyel.lee90 (Talk | contribs) (→Overview) |
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*'''tRNA amber suppressor''' | *'''tRNA amber suppressor''' | ||
**tRNA | **tRNA | ||
| - | S38: in pSB1C3 | + | S38: in pSB1C3 |
**tRNA_TT | S58: in pSB1C3 | **tRNA_TT | S58: in pSB1C3 | ||
**Pveg_spoVG_tRNA | S38 in S24 --- [in progress] | **Pveg_spoVG_tRNA | S38 in S24 --- [in progress] | ||
Revision as of 22:53, 3 September 2011
Danyel
Overview
The constructs involved in the tRNA project so far:
- General information
- tRNA = approx. 100 bp
- T7 amber = approx. 2.7 kb
- GFP amber = approx. 720 bp
- tRNA amber suppressor
- tRNA
S38: in pSB1C3
- tRNA_TT | S58: in pSB1C3
- Pveg_spoVG_tRNA | S38 in S24 --- [in progress]
- Pveg_spoVG_tRNA_TT
- S81 : this contruct in pSB1AK3 (ampR)
- S92 : this contruct in pHM3 (ampR/tetR) --- sequencing required and transformation in subtilis attempted by Adrien.
- pHyperspank_tRNA
- S38 in S82 --- [in progress]
- pHyperspank_tRNA_TT
- S58 in S82 --- [in progress]
- pT7_RBS_GFP_TT :
- S79 : this construct in ampR plasmid (pSB1AK3?).
- functionality has been tested through transformation in BL21 and microscopy. (cf.27/08/11)
- Proteins with amber mutations
- T7 amber_TT
- S80 : in pSB1AK3
