Team:Paris Liliane Bettencourt/Notebook/2011/08/05

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== Cyrille ==
 
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The bands extracted yesterday will be completed and digested using Taq ploymerase. We will use the the Taq polymerase mastermix.
 
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The protocol will be:
 
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25µL of Fermentas PCR mastermix (2x)
 
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25µL DNA template
 
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The PCR cycles will be:
 
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* 80°C 1 min
 
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* 80°C 30 sec
 
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* 55°C 30 sec
 
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* 72°C 30 sec
 
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-> 10 cycles
 
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* 72°C 5 min
 
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* 4°C forever
 

Latest revision as of 14:03, 5 August 2011

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