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Team:Paris Liliane Bettencourt/Notebook/2011/08/05
From 2011.igem.org
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* 72°C 5 min | * 72°C 5 min | ||
* 4°C forever | * 4°C forever | ||
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| + | The PCR product is treated with the PCR product purification kit. | ||
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| + | Then the final product is ligated using T4 DNA ligate. | ||
Revision as of 10:32, 5 August 2011
Cyrille
The bands extracted yesterday will be completed and digested using Taq ploymerase. We will use the the Taq polymerase mastermix.
The protocol will be:
25µL of Fermentas PCR mastermix (2x) 25µL DNA template
The PCR cycles will be:
- 80°C 1 min
- 80°C 30 sec
- 55°C 30 sec
- 72°C 30 sec
-> 10 cycles
- 72°C 5 min
- 4°C forever
The PCR product is treated with the PCR product purification kit.
Then the final product is ligated using T4 DNA ligate.
