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Team:Paris Liliane Bettencourt/Notebook/2011/08/05
From 2011.igem.org
(Difference between revisions)
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The protocol will be: | The protocol will be: | ||
| + | |||
| + | 25µL of Fermentas PCR mastermix (2x) | ||
| + | 25µL DNA template | ||
| + | |||
| + | The PCR cycles will be: | ||
| + | |||
| + | * 80°C 1 min | ||
| + | |||
| + | * 80°C 30 sec | ||
| + | * 55°C 30 sec | ||
| + | * 72°C 30 sec | ||
| + | -> 10 cycles | ||
| + | |||
| + | * 72°C 5 min | ||
| + | * 4°C forever | ||
Revision as of 09:53, 5 August 2011
Cyrille
The bands extracted yesterday will be completed and digested using Taq ploymerase. We will use the the Taq polymerase mastermix.
The protocol will be:
25µL of Fermentas PCR mastermix (2x) 25µL DNA template
The PCR cycles will be:
- 80°C 1 min
- 80°C 30 sec
- 55°C 30 sec
- 72°C 30 sec
-> 10 cycles
- 72°C 5 min
- 4°C forever
