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Team:Paris Liliane Bettencourt/Notebook/2011/08/02/
From 2011.igem.org
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Results of electroporation: IT WORKED after three trials! | Results of electroporation: IT WORKED after three trials! | ||
*Positive control worked | *Positive control worked | ||
| - | *Negative controls didn't show any colonies except when put in the presence of TetR (qty:12,5 µg/mL) which was insufficient => I will re-plate the improbable survivors on plates of different tetracyclin concentrations to test resistance. | + | *Negative controls didn't show any colonies except when put in the presence of TetR (qty:12,5 µg/mL or 5µg/mL) which was insufficient => I will re-plate the improbable survivors on plates of different tetracyclin concentrations to test resistance. |
* Cm resistant strain have survived in low quatities at both 30 and 5 µg/mL of antibiotics | * Cm resistant strain have survived in low quatities at both 30 and 5 µg/mL of antibiotics | ||
*It seems that even cuvettes that had an electric arc were able to produce a couple colonies, which means that it doesn't all the bacteria. | *It seems that even cuvettes that had an electric arc were able to produce a couple colonies, which means that it doesn't all the bacteria. | ||
*To come: protocol and table of results | *To come: protocol and table of results | ||
Revision as of 10:02, 2 August 2011
Adrien Lhomme-Duchadeuil
First, an estimation of how much antibiotics is necessary for Bacillus subtilis. Appropriate concentration in antibiotics
- Chloramphemicol : 5-10 µg/mL
- Spec : 50-100 µg/mL
- Tetracyclin: 25 µg/mL
- Kanamycin: 5µg/mL
- Evm(?): 1µg/mL + 25 µg/mL lincomycin (Verify spelling)
Results of electroporation: IT WORKED after three trials!
- Positive control worked
- Negative controls didn't show any colonies except when put in the presence of TetR (qty:12,5 µg/mL or 5µg/mL) which was insufficient => I will re-plate the improbable survivors on plates of different tetracyclin concentrations to test resistance.
- Cm resistant strain have survived in low quatities at both 30 and 5 µg/mL of antibiotics
- It seems that even cuvettes that had an electric arc were able to produce a couple colonies, which means that it doesn't all the bacteria.
- To come: protocol and table of results
