Team:Paris Liliane Bettencourt/Notebook/2011/07/18/

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Edward

Minipreps

on BBa_I712074 (pT7), concentration of DNA=249.5ng/µL
on BBa_K145001 (T7 RNA polymerase), concentration of DNA=220.4ng/µL
on BBa_K143021 (RBS-spoVg colony 17), concentration of DNA=165.9ng/µL

Restriction Digest of RBS-spoVg and T7 RNA polymerase
- BBa_K145001 cut by XbaI and PstI
- BBa_K143021 cut by SpeI and PstI
  
  Final volume : 20 µL
  DNA Gel electrophoresis (T7 RNA polymerase)
  - 1kb ladder
  - T7 undigested plasmid
  - Digested T7 (BBa_K145001 cut by XbaI and PstI)
  ladder - non digested T7 plasmid - digested T7
  
  ladder - non digested T7 plasmid - digested T7

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