Team:Paris Liliane Bettencourt/Notebook/2011/07/18/

From 2011.igem.org

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</p><p style="display:block;"><a href="/wiki/Image:T7 electro 20072011.jpeg" class="image" title="T7 electro 20072011.jpeg"><img alt="T7 electro 20072011.jpeg" src="/images/9/99/Electrophoresis_090710.jpg" width="425" height="539" border="0" /></a>
[[File:T7 electro 20072011.jpeg]]
[[File:T7 electro 20072011.jpeg]]

Revision as of 13:55, 4 August 2011

Edward

Minipreps

  • on BBa_I712074 (pT7), concentration of DNA=249.5ng/µL
  • on BBa_K145001 (T7 RNA polymerase), concentration of DNA=220.4ng/µL
  • on BBa_K143021 (RBS-spoVg colony 17), concentration of DNA=165.9ng/µL


    Restriction Digest of RBS-spoVg and T7 RNA polymerase

    • BBa_K145001 cut by XbaI and PstI
    • BBa_K143021 cut by SpeI and PstI


      Final volume : 20 µL

      </p><p style="display:block;">DNA Gel electrophoresis (T7 RNA polymerase) </p><p style="display:block;">Protocol (6 wells) </p>

      • 1kb ladder
      • T7 undigested plasmid
      • Digested T7 (BBa_K145001 cut by XbaI and PstI)

      </p><p style="display:block;"><a href="/wiki/Image:T7 electro 20072011.jpeg" class="image" title="T7 electro 20072011.jpeg"><img alt="T7 electro 20072011.jpeg" src="/images/9/99/Electrophoresis_090710.jpg" width="425" height="539" border="0" /></a>

      T7 electro 20072011.jpeg