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    • The basics
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    • T7 polymerase diffusion
    • tRNA diffusion
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    • Microscopy
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    • Integration in B.subtilis
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Data page

The YFP concentration system

Data for our favorite new parts

1. Main Page - YFP:tetR recombinant fusion protein, BBa_K606025: It's a recombinant fusion protein composed of Yellow Fluorescent Protein (YFP) and Tetracycline Repressor Protein (tetR) that can bind to the tet operator sequence (tetO).
2. Main Page - TetO Array, BBa_K606026: 10kb of Tet operator sequence. YFP:tetR will bind to it and concentrate the fluorescence.
3. Main Page - pVeg+SpoVG- YFP:tetR- Double Terminator , BBa_K606027: The YFP:tetR expression system.

Data for Pre-existing Parts

1. Main Page - pVeg + SpoVG, BBa_K143053: promoter and RBS for B. subtilis (Imperial College, iGEM 2008)
2. Main Page - Double standard terminator, BBa_B0015: standard double terminator
   • See the design
   • See the experiments
   • See the nanotubes experiments

   The T7 polymerase diffusion system

Data for our favorite new parts

1. Experience - pVeg SpoVG T7 SpoVG RFP double terminator, BBa_K606039: T7 and RFP under constitutive promoter pVeg + SpoVG, emitter system to “send” the T7 polymerase.
2. Experience - pT7 SpoVG T7 SpoVG GFP T7terminator, BBa_K606036: T7 and GFP under a pT7 promoter to response a emission of T7.

• See the design
• See the model
• See the experiments
• See the nanotubes experiments

The tRNA Amber diffusion system

Data for our favorite new parts

1. Main Page - Promoter Hyperspank, BBa_K606040: IPTG Inducible promoter shortened compared to the one that was before in the registry.
2. Main Page - pHyperspank Tyrosine amber supressor tRNA double terminator, BBa_K606041: system for using the tRNA in a IPTG inducible system.
3. Main Page - Tyrosine amber supressor tRNA for subtilis, BBa_K606034: tRNA tyrosine amber compatible with both B. subtilis and E. coli.
4. Main Page - T7 amber, BBa_K606032: This T7 polymerase contains two amber mutation to be a good receiver in a emitter/receiver system with a Tyrosine tRNA amber.
5. Main Page - T7 amber with autoamplification loop, BBa_K606064
6. Main Page - IPTG inducible tRNA Amber emitter with RFP monitor, BBa_K606065
7. Main Page - Upstream LacI expression IPTG inducible tRNA emitter with RFP Monitor, BBa_K606067

Data for Pre-existing Parts

1. Main Page - pVeg + SpoVG, BBa_K143053: promoter and RBS for B. subtilis (Imperial College, iGEM 2008)
2. Main Page - Double standard terminator, BBa_B0015

• See the design
• See the model
• See the experiments

The ComS diffusion system

Data for our favorite new parts

1. Main Page - ComS gene, BBa_K606037: The comS gene can trigger the MecA/ComS/ComK system. The start and stop codon has been changed for more standard codons.
2. Main Page - ComS gene Codon optimized, BBa_K606038: This is the same gene than the previous one, but it has been synthetized de novo.

Data for Pre-existing Parts

1. Main Page - pVeg + SpoVG, BBa_K143053: promoter and RBS for B. subtilis (Imperial College, iGEM 2008)
2. Main Page - Double standard terminator, BBa_B0015: standard double terminator
3. Main Page - RFP, BBa_K143021: RFP m-cherry.
4. Elowitz's pComG-CFP/pComS-YFP reporter strain - Competence reporter strain. Published in this paper [1]
5. CodY- strain - From Abraham L. Sonenshein's laboratory, Tufts university [2]

• See the design
• See the model
• See the experiments

The SinOp system

Data for our favorite new parts

1. Main Page - KinA gene, BBa_K606046 KinA gene. SinOp kinase.
2. Main Page - pVeg-SpoVg-KinA gene, BBa_K606047 KinA gene with B. subtilis promoter

Data for Pre-existing Parts

1. SinOp reporter strain, given by J.W. Veening, published in this paper [3]

• See the design
• See the experiments

The lambda switch system

Data for our favorite new parts

1. Main Page - pVeg SpoVg CI TT, BBa_K606044: Expression casette for for CI in B. subtilis.
2. Main Page - pVeg SpoVg CI SpoVG RFP TT, BBa_K606068: Expression casette for for CI in B. subtilis with RFP monitor.

Data for Pre-existing Parts

1. Main Page - pVeg + SpoVG, BBa_K143053: promoter and RBS for B. subtilis (Imperial College, iGEM 2008)
2. Main Page - Double standard terminator, BBa_B0015: standard double terminator
3. Main Page - CI, BBa_B0052 - CI gene from the lambda phage.

• See the design

GFP diffusion

Data for Pre-existing Parts

1. Experience - Pveg-spoVG GFP expression construct, BBa_K143079 (Imperial College, iGEM 2008)