Team:Panama/Protocols/Wetlab

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Wetlab

1. Agarose gel electrophoresis

2. Transformation

4. Pgem Cloning

5. Preparing chemically competent cells

6. LB media recipe

7. Ampicillin stock

8.Chloramphenicol stock

9. Mutagenesis PCR

10. Mutagenesis transformation

11. Miniprep Quiagen Kit

12. Miniprep "Dirty"

13. PCR purification

14. Glycerol Stock

15. PCR

16. Agar plates

17. Primer aliquots

18. "Overnight" Cell Culture

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