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Team:Panama/Protocols/Wetlab
From 2011.igem.org
(Difference between revisions)
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| - | 1. [[ | + | 1. [[Agarose gel electrophoresis]] |
| - | 2. | + | 2. [[Transformation]] |
| - | 3. Colony PCR | + | 3. [[Colony PCR]] |
| - | 4. Pgem Cloning | + | 4. [[Pgem Cloning]] |
| - | 5. Preparing chemically competent cells | + | 5. [[Preparing chemically competent cells]] |
| - | 6. LB media | + | 6. [[LB media recipe]] |
| - | 7. | + | 7. [[Ampicillin stock]] |
| - | 8. | + | 8.[[Chloramphenico stock]] |
| - | 9. Mutagenesis PCR | + | 9. [[Mutagenesis PCR]] |
| - | 10. Mutagenesis transformation | + | 10. [[Mutagenesis transformation]] |
| - | 11. Miniprep Quiagen Kit | + | 11. [[Miniprep Quiagen Kit]] |
| - | 12. Miniprep "Dirty" | + | 12. [[Miniprep "Dirty"]] |
| - | 13. PCR purification | + | 13. [[PCR purification]] |
| - | 14. | + | 14. [[Glycerol Stock]] |
| - | 15. | + | 15. [[PCR]] |
| - | 16. Agar plates | + | 16. [[Agar plates]] |
| - | 17. Primer aliquots | + | 17. [[Primer aliquots]] |
| - | 18. Cell Culture | + | 18. [[Cell Culture]] |
[[File:home.jpg|100px|link=https://2011.igem.org/Team:Panama]] | [[File:home.jpg|100px|link=https://2011.igem.org/Team:Panama]] | ||
Revision as of 06:58, 28 September 2011
1. Agarose gel electrophoresis
3. Colony PCR
4. Pgem Cloning
5. Preparing chemically competent cells
10. Mutagenesis transformation
12. Miniprep "Dirty"
13. PCR purification
14. Glycerol Stock
15. PCR
16. Agar plates
17. Primer aliquots
18. Cell Culture
