Team:Panama/Protocols/Wetlab

From 2011.igem.org

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{{:Team:Panama/template|title=Wetlab}}
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1. [[Agarose gel electrophoresis]]
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1. Transformation
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2. [[Transformation]]
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2. PCR
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3. Colony PCR
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3. [[Colony PCR]]
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4. Pgem Cloning
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5. Preparing chemically competent cells
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4. [[Pgem Cloning]]
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6. LB media recipie
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7. Ampicilyn stock
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5. [[Preparing chemically competent cells]]
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8. Chloramfenicol Stock
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9. Mutagenesis PCR
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6. [[LB media recipe]]
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10. Mutagenesis transformation
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11. Miniprep Quiagen Kit
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7. [[Ampicillin stock]]
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12. Miniprep "Dirty"
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13. PCR purification
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8.[[Chloramphenicol stock]]
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14. Glicerol Stock
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15. Agarose gel electrophorsis
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9. [[Mutagenesis PCR]]
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16. Agar plates
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17. Primer aliquots
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10. [[Mutagenesis transformation]]
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18. Cell Culture
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11. [[Miniprep Quiagen Kit]]
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12. [[Miniprep "Dirty"]]
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13. [[PCR purification]]
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14. [[Glycerol Stock]]
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15. [[PCR]]
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16. [[Agar plates]]
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17. [[Primer aliquots]]
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18. [[ "Overnight" Cell Culture]]
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19. [[NZY+ Recipe]]
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20. [[Gel DNA extraction]]
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21. [[SOC Recipe]]
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[[File:home.jpg|100px|link=https://2011.igem.org/Team:Panama]]

Latest revision as of 01:46, 29 September 2011