Team:Panama/Protocols/Wetlab

From 2011.igem.org

(Difference between revisions)
(Created page with " 1. Transformation 2. PCR 3. Colony PCR 4. Pgem Cloning 5. Preparing chemically competent cells 6. LB media recipie 7. Ampicilyn stock 8. Chloramfenicol Stock 9. Mutagenesis PCR ...")
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1. Transformation
1. Transformation
 +
2. PCR
2. PCR
 +
3. Colony PCR
3. Colony PCR
 +
4. Pgem Cloning
4. Pgem Cloning
 +
5. Preparing chemically competent cells
5. Preparing chemically competent cells
 +
6. LB media recipie
6. LB media recipie
 +
7. Ampicilyn stock
7. Ampicilyn stock
 +
8. Chloramfenicol Stock
8. Chloramfenicol Stock
 +
9. Mutagenesis PCR
9. Mutagenesis PCR
 +
10. Mutagenesis transformation
10. Mutagenesis transformation
 +
11. Miniprep Quiagen Kit
11. Miniprep Quiagen Kit
 +
12. Miniprep "Dirty"
12. Miniprep "Dirty"
 +
13. PCR purification
13. PCR purification
 +
14. Glicerol Stock
14. Glicerol Stock
 +
15. Agarose gel electrophorsis
15. Agarose gel electrophorsis
 +
16. Agar plates
16. Agar plates
 +
17. Primer aliquots
17. Primer aliquots
 +
18. Cell Culture
18. Cell Culture

Revision as of 05:42, 28 September 2011

1. Transformation

2. PCR

3. Colony PCR

4. Pgem Cloning

5. Preparing chemically competent cells

6. LB media recipie

7. Ampicilyn stock

8. Chloramfenicol Stock

9. Mutagenesis PCR

10. Mutagenesis transformation

11. Miniprep Quiagen Kit

12. Miniprep "Dirty"

13. PCR purification

14. Glicerol Stock

15. Agarose gel electrophorsis

16. Agar plates

17. Primer aliquots

18. Cell Culture