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Protocols

How we design and build our BioBrick parts?

Rational Design

We need to build an expression platform that works into our modified E.coli. For this, we choose four standardized biological parts from the Registry, those parts are: Promoter T7, Ribosomal Binding Site (RBS), GFP Reporter and Terminator; Were selected using rational design abstraction from molecular biology and genetic engineering to enable us to build up rationally-engineered, our expression platform.

The rationally-egineering for ligate the BioBrick parts was able by using the[http://partsregistry.org/Assembly:3A_Assembly 3A Assembly method]