Team:Panama/8 September 2011

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(Created page with "We did an electrophoresis of the miniprep´s products (9/4/11) and PCR´s products (9/5/11). Trying to probe the primer´s couples, we run a P. aeruginosa genomic DNA´s PCR wit...")
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We did an electrophoresis of the miniprep´s products (9/4/11) and PCR´s products (9/5/11).
 
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Trying to probe the primer´s couples, we run a P. aeruginosa genomic DNA´s PCR with the following primer´s combinations:
 
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• RhTf1b+RhT2b
 
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• RhTR2a+RhTf1a
 
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• RhTBiof1b+RhTBio2b
 
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• RhTbioR2a+RhTbiof1a
 
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[[File:tabla4.png|700px|thumb|left|alt text]]
 
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Volume of master mix: 12.5 uL
 
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Volume of DNA: 2 uL
 
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Volume of Primers: 2.5 uL everyone
 
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Volume of H2O: 6.5 uL
 
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Total Volume of reaction: 26 uL
 
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The most efficient primers were: RhTf1b+RhT2b and RhTbiof1b+RhTbio2b
 

Latest revision as of 03:30, 29 September 2011

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