From 2011.igem.org
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| - | We prepared 5 mL of chloramphenicol and 5 mL of ampicilin, the chloramphenicol was stored on -20 centigrades and the ampicilin was stored on 4 centigrades. | + | We did an electrophoresis with the Platinum blue master mix using the primer RhTf1b+RhT2b and the 4 tubes with Pseudomonas aeruginosa's genomic DNA. |
| - | We prepared 14 petri dishes with LB media, 11 petri dishes with ampicilin and 3 petri dishes with chloramphenicol.
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| - | We inoculate 2 petri dishes and a negative control, with the inducible promoter, but with three different E. coli's strains: E. coli K-12, E. coli JM109 (competent with CaCl2) and E. coli JM109 (competent with MgCl2).\
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| - | Also, we inoculate 2 petri dishes with E. coli k-12 and E. coli JM109 competent cells, without transformation, (trying to probe if someone of these strains have ampicilin's resistance).
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| - | We inoculate another three petri dishes with E. coli k-12, and E. coli JM109, without transformation, (trying to probe if someone of these strains have ampicilin's resistance, and in the third petri dish we inoculate the inducible promoter.
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| - | The objectives of the day are:
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| - | Which cell is competent?
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| - | Which strain has ampicilin or chloramphenicol's resistance, without transformation?
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| - | Do the chloramphenicol works?
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Latest revision as of 03:48, 29 September 2011
We did an electrophoresis with the Platinum blue master mix using the primer RhTf1b+RhT2b and the 4 tubes with Pseudomonas aeruginosa's genomic DNA.
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