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Team:Osaka/Tests
From 2011.igem.org
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== Test == | == Test == | ||
| - | the cells were plated on respective agar plates at different dilutions, air dried, and then exposed to different doses of UV radiation | + | === Cell survival assay === |
| + | the cells were plated on respective agar plates at different dilutions, air dried, and then exposed to different doses of UV radiation. Plates were wrapped with aluminum foil and incubated in the dark. Colonyforming | ||
| + | units were scored after 16h incubation at 37°C. | ||
| + | |||
| + | PprI,PprA,PprM,RecAをそれぞれ導入した大腸菌にUV照射 | ||
Revision as of 10:28, 4 October 2011
Contents |
Test
Cell survival assay
the cells were plated on respective agar plates at different dilutions, air dried, and then exposed to different doses of UV radiation. Plates were wrapped with aluminum foil and incubated in the dark. Colonyforming units were scored after 16h incubation at 37°C.
PprI,PprA,PprM,RecAをそれぞれ導入した大腸菌にUV照射
Future work
We created some parts (PprI , PprA , PprM , RecA) but did not have time to evaluate them. Also, devices containing 2 or more DNA repair gene should have been constructed and assayed.
Reference
PprA: a protein implicated in radioresistance of Deinococcus radiodurans stimulates catalase activity in Escherichia coli, Swathi Kota et al (2006)
