Team:Northwestern/Notebook/Week11

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RETURN TO IGEM 2010



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Day 49 - Monday, August 22nd 2011

  • More extensive minipreps - this time of all the genomic promoter ligations.
  • Plated our glycerol stocks of the successful gels from friday to make sure everything worked properly.
  • Ran a plate of our controls at the high-throughput lab.
  • Digested and ran the genomic promoter ligations on a gel


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Day 50 - Tuesday, August 23nd 2011

  • Analyzed the results from our control plate at the high throughput lab.
  • We wanted to continue testing, but we were having trouble getting all of our samples to grow properly.
  • Prepared 384 well plate layouts for future tests.


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Day 51 - Wednesday, August 24nd 2011

  • The bacteria continued to have very weird growth patterns. We tried two other spectrophotometers to try and judge if ours was malfunctioning.
  • We eventually decided that our machine was okay, but that we had not been mixing the samples properly before reading them, which led to our weird results.
  • We made new M9 media in case that was causing the growth problems.


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Day 52 - Thursday, August 25nd 2011

  • Ran a 96 well plate with our controls and GFP samples. We added the autoinducer to all of the samples at an OD of approximately 0.3
  • Began setting up interviews and a script for our human practices video


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Day 53 - Friday, August 26nd 2011

  • Analyzed the results from our GFP test.
  • Continued working on human practices