We used [http://www.neb.com/nebecomm/products/productM0531.asp Phusion High Fidelity PCR Master Mix] from New England Biolabs.

Assemble all reaction components on ice and quickly transfer the reactions to a thermocycler preheated to the denaturation temperature (98 degrees C). All components should be mixed and centrifuged prior to use. It is important to add Phusion Master Mix last in order to prevent any primer degredation.

For 50 uL reaction:

10 uM forward primer	2.5 uL
10 uM reverse primer	2.5 μL
Template DNA	variable
Nuclease-free water	to final volume (including mix) of 50 μL
2X Phusion Master Mix	25 μL