Broths LB Broth

1. To 950 mL of deionized water, add:
   • Tryptone 10 g
   • Yeast Extract 5 g
   • NaCl 10 g
2. Fill to 1L. Autoclave for 1 hour.

SOB

1. To 950 mL of deionized water, add:
   • Tryptone 20 g
   • Yeast Extarct 5 g
   • NaCl 0.5 g
2. Shake until the solutes have dissolved. Add 10 mL of a 250 mM solution of KCl (dissolve 0.465 g KCl in 25 mL water). adjust pH to 7.0 using NaOH. Adjust the volume of the solution to 1 liter with deionized water. Sterilize by autoclaving for 20 minutes.
3. Just before use, add 5 mL of a sterile solution of 2 M MgCl2, which can be made by dissolving 19 g of anhydrous MgCl2 in 90 mL of deionized water (adjust mass accordingly if using MgCl2 hexahydrate) and autoclaving for 20 minutes.

SOC

SOC medium is identical to SOB medium, except it also contains 20 mL glucose. After the SOB medium has been autoclaved, allow it to cool to 60 degrees or less and then add 20 mL of a sterile 1 M solution of glucose (dissolve 90 mL of dI water, adjust volume to 100 mL, then filter sterilize).