From 2011.igem.org
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| - | __NOTOC__
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| - | {{:Team:Northwestern/Templates/trial}}
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| - | <div id="header" style="margin: 14px 0px 0px -0px;">
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| - | <img src="https://static.igem.org/mediawiki/2011/e/eb/Heading_banner_long2.gif" height = "70px" width="750px" style="opacity:1;filter:alpha(opacity=100)" alt="NU-igem banner"/ border="0">
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| - | <div style="margin: -55px 0px 0px 80px;font:35px helvetica; color:#ffffff;"> Parts</div>
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| - | <div style="margin: -40px 0px 0px 400px;font:35px helvetica; color:#444444;"> Characterization</div>
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| - | <br>
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| - | The characterization of our parts was a long and detailed process involving weeks of testing and analysis. However, the general process was similar for most of our samples:
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| - | 1) The night before testing, grow overnight cultures of all samples and controls in M9 Media.
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| - | 2) In the morning, dilute all density to an optical density of 0.05.
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| - | 3) Let grow for approximately 3-4 hours, monitoring the OD to make sure it does not grow above 0.5.
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| - | 4) When the OD of the samples reaches 0.5, distribute the samples into a 96 or 384 well plate.
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| - | 5) Simultaneously induce with PAI1 and/or PAI2 using a 96 to 384 well plate distributor.
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| - | 6) Put the plate a Synergy Fluorescence reader, take regular measurements of OD and Fluorescence. For GFP, we used an excitation frequency of 485nm and an emission frequency of 528nm. For RFP, we used an excitation frequency of 580nm and an emission frequency of 610nm.
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Latest revision as of 02:33, 26 September 2011
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