Team:Northwestern

From 2011.igem.org

(Difference between revisions)
 
(22 intermediate revisions not shown)
Line 6: Line 6:
<html>
<html>
-
<div id="header" style="margin: -8px 0px 0px -0px;">
+
<DIV id="header" style="margin: -8px 0px 0px -0px;">
-
<img src="https://static.igem.org/mediawiki/2011/b/bf/Heading_banner3.gif" height = "auto" width="450px" style="opacity:1;filter:alpha(opacity=100)" alt="NU-igem logo"/ border="0">
+
<img src="https://static.igem.org/mediawiki/2011/b/bf/Heading_banner3.gif" height = "60px" width="450px" style="opacity:1;filter:alpha(opacity=100)" alt="NU-igem logo"/ border="0">
<div style="margin: -50px 0px 0px 120px;font:35px helvetica; color:#ffffff;"> Abstract
<div style="margin: -50px 0px 0px 120px;font:35px helvetica; color:#ffffff;"> Abstract
-
</div></div>
+
</DIV></DIV>
</html>
</html>
Line 19: Line 19:
<br />
<br />
<DIV style="font:14px Helvetica;width:450px; height:auto;">
<DIV style="font:14px Helvetica;width:450px; height:auto;">
-
''Pseudomonas aeruginosa'' is an opportunistic pathogen that colonizes in immunocompromised patients. It is commonly found within hospitals and is the predominant microorganism responsible for chronic lung infections in patients with cystic fibrosis. The bacterium also causes 12% of hospital-acquired urinary tract infections, 10% of bloodstream infections, and 8% of surgical wound infections. Existing detection methods utilize expensive, time consuming blood and cell cultures. Our goal is to transplant elements of the organism’s natural quorum sensing system into ''E. coli'' in order to create a novel sensor that is able to detect the presence of this bacterium within a blood sample both quickly and effectively.
+
<center>'''My N.U. P.A.L'''<br>'''Northwestern University's ''Pseudomonas aeruginosa'' Locator'''</center><br>
 +
 
 +
''Pseudomonas aeruginosa'' is an opportunistic pathogen commonly found in immunocompromised patients. In addition to being the primary cause of lung infections in cystic fibrosis patients, many severe nosocomial infections can be attributed to ''P. aeruginosa''. Currently, the standard detection method requires a potential sample to be grown overnight and then screened for the pathogen of interest. We have engineered an ''E. coli''-based biosensor capable of detecting the presence of autoinducer molecules unique to ''P. aeruginosa''. Thus, our system provides a faster detection method without sacrificing reliability or experimental resolution. Quorum sensing in ''P. aeruginosa'' is a complex hierarchy that governs the expression of numerous virulence genes. To realize our objective, we harnessed the native cell signaling and quorum sensing machinery of ''P. aeruginosa''. We have thus created a novel, inexpensive biosensor capable of detecting the presence of ''P. aeruginosa'' both quickly and effectively.
</DIV>  
</DIV>  
-
<br>
 
-
'''[[Team:Northwestern/Project/Introduction|Project Description]] (more detailed)'''
 
-
 
-
'''[[Team:Northwestern/Safety|Safety Proposal]]'''
 
<br>
<br>
Line 32: Line 30:
<html><div id="return-to-igem" style="color:#ffffff;background:#333333;padding: 30px 10px 10px 10px;margin: 0px 0px 0px 30px; font:25px helvetica; width:650px;">   
<html><div id="return-to-igem" style="color:#ffffff;background:#333333;padding: 30px 10px 10px 10px;margin: 0px 0px 0px 30px; font:25px helvetica; width:650px;">   
&nbsp; &nbsp; &nbsp; &nbsp;iGEM Workers Researching on a Crossbeam 1932
&nbsp; &nbsp; &nbsp; &nbsp;iGEM Workers Researching on a Crossbeam 1932
-
<img src="https://static.igem.org/mediawiki/2011/1/15/Volkswagen_crafter_girder.jpg" width="650" height="auto" alt="group" />
+
<img src="https://static.igem.org/mediawiki/2011/1/15/Volkswagen_crafter_girder.jpg" width="650" height="450px" alt="group" />
<div style="text-align:right; font-size:15px;">(Based on the original <a href="https://static.igem.org/mediawiki/2011/4/4a/Construction_Workers.jpg"><i>Construction Workers Lunching on a Crossbeam</i></a>)
<div style="text-align:right; font-size:15px;">(Based on the original <a href="https://static.igem.org/mediawiki/2011/4/4a/Construction_Workers.jpg"><i>Construction Workers Lunching on a Crossbeam</i></a>)
</div><br></div></html>
</div><br></div></html>
-
----------------------
+
 
-
[[Image:Sponsor_northwestern.jpg]]
+
 
-
[[Image:Sponsor_weinberg.jpg]]
+
{{:Team:Northwestern/Templates/footer}}
-
[[Image:Sponsor_mccormick.jpg]]
+

Latest revision as of 02:02, 29 October 2011

RETURN TO IGEM 2010


iGEM_NU
    twitter


    My N.U. P.A.L
    Northwestern University's Pseudomonas aeruginosa Locator

    Pseudomonas aeruginosa is an opportunistic pathogen commonly found in immunocompromised patients. In addition to being the primary cause of lung infections in cystic fibrosis patients, many severe nosocomial infections can be attributed to P. aeruginosa. Currently, the standard detection method requires a potential sample to be grown overnight and then screened for the pathogen of interest. We have engineered an E. coli-based biosensor capable of detecting the presence of autoinducer molecules unique to P. aeruginosa. Thus, our system provides a faster detection method without sacrificing reliability or experimental resolution. Quorum sensing in P. aeruginosa is a complex hierarchy that governs the expression of numerous virulence genes. To realize our objective, we harnessed the native cell signaling and quorum sensing machinery of P. aeruginosa. We have thus created a novel, inexpensive biosensor capable of detecting the presence of P. aeruginosa both quickly and effectively.




           iGEM Workers Researching on a Crossbeam 1932 group



    Sponsor northwestern.jpg Sponsor weinberg.jpg Sponsor mccormick.jpg