Team:NYC Wetware/Notebook/Week9

From 2011.igem.org

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7/18/2011<br/>
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Iterative transformation, inoculation, miniprep. Prepared 20+ E. coli genes for assembly into our radioresistance biobricks.  
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Ran gel on the final 10 E. coli genes that still needed extraction. Separation and use of transilluminator successful. Extracted bands of interest.
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Ran colony PCR to test out our new D. rad primers. <br/>
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7/19/2011<br/>
 
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Yesterday's D. rad colony PCR FAILED. Re-runs with extracted DNA from early-summer trials and with higher concentration of colonies. Re-runs FAIL too.<br/>
 
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Extracted DNA from yesterdays gels and ligated them into plasmid for transformation tomorrow. <br/>
 
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Also, looked into using the screening plasmid, pSB1A10, for future transformations and how to correctly use the pBAD promoter.

Revision as of 02:50, 28 September 2011