Team:NYC Wetware/Notebook/Week7

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7/5/11<br/>
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PCR test to see if all of our 27 E. coli primers are working. Most seem pretty good.  
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PCR test to see if all of our 27 E. coli primers are working. Most seem pretty good. See our impressive-looking results: Row 1: [[1]] Row 2: [[2]]
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Successfully extracted our first 17 E. coli genes for biobricking from all the E. coli PCR reactions that yielded a single product band. Nanodrop results confirm DNA extracted. The PCR reactions with multiple product bands were rerun on a gel and the gene product of interest was excised on our new transilluminator.
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Inserted our 17 successfully extracted genes of interest into plasmid pSB1C3. Lengthy, tricky process. Will be running gel to confirm we've made well-formed biobricks. Then, on to transformation!
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10 Remaining unisolated E. coli genes ran in gel, but it was thoroughly incomprehensible. WIll try again.

Latest revision as of 02:43, 28 September 2011