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Team:NYC Wetware/BioNumbers
From 2011.igem.org
There’s a Gene for That
Deinococcus Radioduran (D. rad) is famous for being resistant to 3000 times the radiation dose lethal to humans. Inspired to prove the potential of genetic engineering, the New York City iGEM Team chose to create radiation-resistant biobricks. We planned to find the genes that give D. rad extraordinary radiation resistance, and put them into loserish bacteria like E. coli, and see if we could turn the E. coli into super-hero bacteria.By finding genes that allow life to thrive in extremely harsh environments, we help develop the emerging field of genetic engineering into a classic engineering disciplines like electrical and mechanical engineering. We will demonstrated that we too can tackle the complex problems. Below find a table of extremophile values. Achieve these and demonstrate the power of synthetic biology to yourselves, fellow iGEM teams, fellow engineers and the general public. In addition to the list below, we have submitted these values to the BioNumbers database, of Harvard Medical School and Weizmann Institute. Good Luck!
- High Radiation: Deinocuccus radiodurans can live in 15000 Gy
Low Temperature: Arthrobacter can live in < -15 celsius
High Temperature: Strain 121 can live in 130 celsius
High Pressure: Some microbes can survive 130 MPa
Vacuum: Some insects, microbes and seeds can live in total vacuum
Salinity (Concentration): Dunaliella salina can live in 2-5 M NaCl
High pH : As yet unnamed can live in 12.8 Low pH: Cyanidium caldarium can survive 0
Either oxygen deficient OR carbon dioxide deficient environments: Chloroflexus aurantacus can live in 0M O2 OR 0M CO2
Low Water Activity :Saccharomyces rouxii can live in 0.62
Longest synthetic genome :Mycoplasma mycoides JCVI-syn1.0 can live in 1.08-mega-base pair
Longest sustained expression of a pore-forming protein in a liposome was 4 Days
Enzyme efficiency of wintergreen-smell producing Biobrick BBa_J45004 (mM-1⋅s-1) in E. coli is 7.65 ± 0.618
