Team:NCTU Formosa

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<div id="banner"><a href = "https://2011.igem.org/Team:NCTU_Formosa"><img src = "https://static.igem.org/mediawiki/2011/8/8c/Banner010.jpg" height="236" width="944"/></a></div>
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   <li><a onClick="out('cm-nav')" class="arrow">Team </a>
   <li><a onClick="out('cm-nav')" class="arrow">Team </a>
       <ul>
       <ul>
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         <li><a href="https://2011.igem.org/Team:NCTU_Formosa/members">Members</a></li>
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         <li><a  
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href="https://2011.igem.org/Team:NCTU_Formosa/members">Members</a></li>
         <li><a href="http://www.nctu.edu.tw/english/index.php">School</a></li>
         <li><a href="http://www.nctu.edu.tw/english/index.php">School</a></li>
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         <li><a href="https://2011.igem.org/Team:NCTU_Formosa/gallery">Gallery</a></li>
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         <li><a  
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href="https://2011.igem.org/Team:NCTU_Formosa/gallery">Gallery</a></li>
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       </ul>
       </ul>
   </li>
   </li>
   <li><a onClick="out('cm-nav')" class="arrow">Project</a>
   <li><a onClick="out('cm-nav')" class="arrow">Project</a>
       <ul class="arrow-pad">
       <ul class="arrow-pad">
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         <li><a href="https://2011.igem.org/Team:NCTU_Formosa/introduction">Introduction</a></li>
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         <li><a  
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         <li><a onClick="out('cm-nav')" class="arrow no-click">RNA Thermometer</a>
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href="https://2011.igem.org/Team:NCTU_Formosa/introduction">Introduction</a></li>
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         <li><a onClick="out('cm-nav')" class="arrow no-click">RNA  
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Thermometer</a>
             <ul>
             <ul>
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               <li><a href="https://2011.igem.org/Team:NCTU_Formosa/RNA_design">Design</a></li>
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               <li><a href="https://2011.igem.org/Team:NCTU_Formosa/RNA_data">Data</a></li>
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               <li><a href="https://2011.igem.org/Team:NCTU_Formosa/RNA_discussion">Modeling</a></li>
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href="https://2011.igem.org/Team:NCTU_Formosa/RNA_design">Design</a></li>
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               <li><a  
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href="https://2011.igem.org/Team:NCTU_Formosa/RNA_data">Data</a></li>
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               <li><a  
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href="https://2011.igem.org/Team:NCTU_Formosa/RNA_discussion">Modeling</a></li>
             </ul>
             </ul>
         </li>
         </li>
         <li><a onClick="out('cm-nav')" class="arrow">CI promoter </a>
         <li><a onClick="out('cm-nav')" class="arrow">CI promoter </a>
             <ul>
             <ul>
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href="https://2011.igem.org/Team:NCTU_Formosa/CI_design">Design</a></li>
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               <li><a  
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href="https://2011.igem.org/Team:NCTU_Formosa/CI_data">Data</a></li>
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               <li><a  
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href="https://2011.igem.org/Team:NCTU_Formosa/CI_discussion">Modeling</a></li>
             </ul>
             </ul>
         </li>
         </li>
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         <li><a onClick="out('cm-nav')" class="arrow">Carotenoid synthesis pathway</a>
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         <li><a onClick="out('cm-nav')" class="arrow">Carotenoid synthesis  
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pathway</a>
             <ul>
             <ul>
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               <li><a href="https://2011.igem.org/Team:NCTU_Formosa/CSP_data">Data</a></li>
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href="https://2011.igem.org/Team:NCTU_Formosa/CSP_design">Design</a></li>
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               <li><a  
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href="https://2011.igem.org/Team:NCTU_Formosa/CSP_data">Data</a></li>
             </ul>
             </ul>
         </li>
         </li>
         <li><a onClick="out('cm-nav')" class="arrow">Butanol pathway</a>
         <li><a onClick="out('cm-nav')" class="arrow">Butanol pathway</a>
             <ul>
             <ul>
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               <li><a href="https://2011.igem.org/Team:NCTU_Formosa/BP_design">Design</a></li>
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               <li><a href="https://2011.igem.org/Team:NCTU_Formosa/BP_data">Data</a></li>
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href="https://2011.igem.org/Team:NCTU_Formosa/BP_design">Design</a></li>
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               <li><a  
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href="https://2011.igem.org/Team:NCTU_Formosa/BP_data">Data</a></li>
             </ul>
             </ul>
         </li>
         </li>
         <li><a onClick="out('cm-nav')" class="arrow">Violacein pathway</a>
         <li><a onClick="out('cm-nav')" class="arrow">Violacein pathway</a>
             <ul>
             <ul>
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               <li><a href="https://2011.igem.org/Team:NCTU_Formosa/VP_design">Design</a></li>
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               <li><a href="https://2011.igem.org/Team:NCTU_Formosa/VP_data">Data</a></li>
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href="https://2011.igem.org/Team:NCTU_Formosa/VP_design">Design</a></li>
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               <li><a  
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href="https://2011.igem.org/Team:NCTU_Formosa/VP_data">Data</a></li>
             </ul>
             </ul>
         </li>         
         </li>         
       </ul>
       </ul>
   </li>
   </li>
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   <li><a href="https://2011.igem.org/Team:NCTU_Formosa/modeling">Measurements</a></li>
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   <li><a  
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href="https://2011.igem.org/Team:NCTU_Formosa/modeling">Measurements</a></li>
   <li><a href="https://2011.igem.org/Team:NCTU_Formosa/parts">Parts</a></li>
   <li><a href="https://2011.igem.org/Team:NCTU_Formosa/parts">Parts</a></li>
   <li><a href="https://2011.igem.org/Team:NCTU_Formosa/safty">Safety</a></li>
   <li><a href="https://2011.igem.org/Team:NCTU_Formosa/safty">Safety</a></li>
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   <li><a href="https://2011.igem.org/Team:NCTU_Formosa/humanpractice">Human Practice</a></li>
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   <li><a href="https://2011.igem.org/Team:NCTU_Formosa/humanpractice">Human  
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  <li><a href="https://2011.igem.org/Team:NCTU_Formosa/contributions">Attribution</a></li>
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Practice</a></li>
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        <li><a  
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href="https://2011.igem.org/Team:NCTU_Formosa/contributions">Attribution</a></li>
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   <li><a onClick="out('cm-nav')" class="arrow no-click">Notebook </a>
   <li><a onClick="out('cm-nav')" class="arrow no-click">Notebook </a>
       <ul>
       <ul>
           <li><a onClick="out('cm-nav')" class="arrow">Protocols</a>
           <li><a onClick="out('cm-nav')" class="arrow">Protocols</a>
             <ul>
             <ul>
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               <li><a href="https://2011.igem.org/Team:NCTU_Formosa/protocol">Mutation</a></li>
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               <li><a href="https://2011.igem.org/Team:NCTU_Formosa/protocol_F">Flow Cytometry</a></li>
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               <li><a href="https://2011.igem.org/Team:NCTU_Formosa/protocol_G">GC</a></li>
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href="https://2011.igem.org/Team:NCTU_Formosa/protocol">Mutation</a></li>
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               <li><a  
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 +
href="https://2011.igem.org/Team:NCTU_Formosa/protocol_F">Flow Cytometry</a></li>
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               <li><a  
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 +
href="https://2011.igem.org/Team:NCTU_Formosa/protocol_G">GC</a></li>
             </ul>
             </ul>
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           <li><a href="https://2011.igem.org/Team:NCTU_Formosa/calendar">Calendar</a></li>
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href="https://2011.igem.org/Team:NCTU_Formosa/calendar">Calendar</a></li>
       </ul>
       </ul>
   </li>
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<div class="fb-like-box" data-href="http://www.facebook.com/pages/2011IGEM-
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NCTU_Formosa/267841893250331" data-width="280" data-show-faces="true" data-
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<div id="blueBox"><p>Concept</p></div>
<div id="blueBox"><p>Concept</p></div>
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<div id="Box1"><center>
<div id="Box1"><center>
<embed z-index="1" width="550" height="400" src="https://static.igem.org/mediawiki/2011/6/6e/5.swf" type="application/x-shockwave-flash" invokeurls="false" allowscriptaccess="never" wmode="transparent" /></center></div>
<embed z-index="1" width="550" height="400" src="https://static.igem.org/mediawiki/2011/6/6e/5.swf" type="application/x-shockwave-flash" invokeurls="false" allowscriptaccess="never" wmode="transparent" /></center></div>
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<div id="blueBox"><p>Achievements</p></div>
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<div id="Box">
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<table>
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<tr>
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<td ><img src="https://static.igem.org/mediawiki/2011/e/ec/Nctu_check.png" width="35"/></td>
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<td><p><a href="https://igem.org/Team_List.cgi?year=2011">Register</a> our team.</p></td>
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</tr>
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<tr>
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  <td><img src="https://static.igem.org/mediawiki/2011/e/ec/Nctu_check.png" width="35"/></td>
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  <td><p>Complete the judging form.</p></td>
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</tr>
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<tr>
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<td ><img src="https://static.igem.org/mediawiki/2011/e/ec/Nctu_check.png" width="35"/></td>
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<td><p>Set up a <a href="https://2011.igem.org/Team:NCTU_Formosa">team wiki</a>.</p></td>
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</tr>
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<tr>
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<td valign="middle"><img src="https://static.igem.org/mediawiki/2011/e/ec/Nctu_check.png" width="35"/></td><td><p>Prepared a poster and a presentation</p></td>
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</tr>
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<tr>
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<td><img src="https://static.igem.org/mediawiki/2011/e/ec/Nctu_check.png" width="35"/></td>
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<td><p>Submit 46 new BioBrick parts.</p></td>
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</tr>
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<tr>
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<td valign="top"><img src="https://static.igem.org/mediawiki/2011/e/ec/Nctu_check.png" width="35"/></td>
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<td><p>Design several new <a href="https://2011.igem.org/Team:NCTU_Formosa/parts">BioBricks</a>, including
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<a href="https://2011.igem.org/Team:NCTU_Formosa/CSP_design">Carotenoid synthesis Pathway</a>, 
 +
<a href="https://2011.igem.org/Team:NCTU_Formosa/VP_design">Violacein biosynthesis pathways</a>, and
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<a href="https://2011.igem.org/Team:NCTU_Formosa/VP_design">Butanol synthesis pathway</a>.The Butanol synthesis pathway can produce Biofuel!</p>
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</td>
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</tr>
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<tr>
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<td><img src="https://static.igem.org/mediawiki/2011/e/ec/Nctu_check.png" width="35"/></td>
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<td><p>Most of our <a href="https://2011.igem.org/Team:NCTU_Formosa/BP_data">BioBricks</a> work as expected.</p></td>
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</tr>
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<tr>
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<td><img src="https://static.igem.org/mediawiki/2011/e/ec/Nctu_check.png" width="35"/></td>
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<td><p>Characterize several <a href="https://2011.igem.org/Team:NCTU_Formosa/parts">BioBricks</a> (<a href="http://partsregistry.org/Part:BBa_K118008:Experience">BBa_K118008</a>, <a href="http://partsregistry.org/Part:BBa_K274004:Experience">BBa_K274004</a>). </p></td>
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</tr>
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<tr>
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<td valign="top"><img src="https://static.igem.org/mediawiki/2011/e/ec/Nctu_check.png" width="35"/></td>
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<td><p>Our favourites are <a href="http://partsregistry.org/Part:BBa_K539691">BBa_K539691</a>, <a href="http://partsregistry.org/Part:BBa_K539151">BBa_K539151</a>, <a href="http://partsregistry.org/Part:BBa_K539281">BBa_K539281</a>, <a href="http://partsregistry.org/Part:BBa_K539461">BBa_K539461</a>,  and <a href="http://partsregistry.org/Part:BBa_K539742">BBa_K539742</a>.</p></td>
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</tr>
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<tr>
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<td><img src="https://static.igem.org/mediawiki/2011/e/ec/Nctu_check.png" width="35"/></td>
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<td><p>Incorporated <a href="https://2011.igem.org/Team:NCTU_Formosa/humanpractice">Human Practices</a> and <a href="https://2011.igem.org/Team:NCTU_Formosa/modeling">modeling</a> into our design. </p></td>
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</tr>
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<tr>
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<td valign="top"><img src="https://static.igem.org/mediawiki/2011/e/ec/Nctu_check.png" width="35"/></td>
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<td><p>Designed <a href="https://2011.igem.org/Team:NCTU_Formosa/introduction">Pathway commander</a>, a novel concept to  to control a pathway to stop at any intermediate state.</p></td>
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</tr>
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</table>
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<br>
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</div>
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<div id="blueBox"><p>Abstract</p></div>
<div id="blueBox"><p>Abstract</p></div>
<div id="Box">
<div id="Box">
<p>
<p>
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As one of the main challenges of in vivo experiments is controlling the flux through a synthetic  metabolic pathway, it’s design lies in selecting well-matched genetic components that when coupled, can reliably produce the desired behavior. Although model equations can calculate parameter values, a challenge still remains in selecting the bio-bricks that can reliably implement a desired cellular function with quantitative values. In previous studies, synthetic biologists have created numerous synthetic circuits; each generating different protein expression levels in order to test performance in reliability and consistency, but this process is both tedious and time consuming. To overcome this problem, our team designed a novel circuit design: method_ Pathway Commander. By this design method, we construct a single version of a synthetic metabolic pathway circuit that can use culture temperature shifts to control the expression levels of a series of metabolic proteins at the precise times. We have implemented the Pathway_Commander design in (1) Carotenoid synthesis Pathway, (2) Violacein biosynthesis pathways and (3) Butanol synthesis pathway in E. coli. This circuit design utilizes a temperature controlled system that gives precision control over metabolic protein expression which amounts to optimal synthesis that can maximize synthesis of a given compound or drug.  
+
As one of the main challenges of in vivo experiments is controlling the flux  
-
</p>
+
 
 +
through a synthetic  metabolic pathway, it’s design lies in selecting well-
 +
 
 +
matched genetic components that when coupled, can reliably produce the desired  
 +
 
 +
behavior. Although model equations can calculate parameter values, a challenge  
 +
 
 +
still remains in selecting the bio-bricks that can reliably implement a desired  
 +
 
 +
cellular function with quantitative values. In previous studies, synthetic  
 +
 
 +
biologists have created numerous synthetic circuits; each generating different  
 +
 
 +
protein expression levels in order to test performance in reliability and  
 +
 
 +
consistency, but this process is both tedious and time consuming. To overcome  
 +
 
 +
this problem, our team designed a novel circuit design: method_ Pathway  
 +
 
 +
Commander. By this design method, we construct a single version of a synthetic  
 +
 
 +
metabolic pathway circuit that can use culture temperature shifts to control the  
 +
 
 +
expression levels of a series of metabolic proteins at the precise times. We have  
 +
 
 +
implemented the Pathway_Commander design in (1) Carotenoid synthesis Pathway, (2)  
 +
 
 +
Violacein biosynthesis pathways and (3) Butanol synthesis pathway in E. coli.  
 +
 
 +
This circuit design utilizes a temperature controlled system that gives precision  
 +
 
 +
control over metabolic protein expression which amounts to optimize the synthesis that  
 +
 
 +
can maximize synthesis of a given compound or drug.  
 +
</p><br><br>
 +
 
 +
 
 +
<center><div class="lin"><b>
 +
 
 +
 
 +
 
 +
<a href="https://2011.igem.org/Team:NCTU_Formosa/humanpractice"><img
 +
 
 +
src="https://static.igem.org/mediawiki/2011/f/f3/Human_practice.jpg" alt="" height="50"
 +
 
 +
/></a>
 +
Human Practice
 +
<a href="https://2011.igem.org/Team:NCTU_Formosa/members"><img
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 +
src="https://static.igem.org/mediawiki/2011/1/1a/NctuTeam.jpg" alt="" height="50"
 +
 
 +
/></a>
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Members
 +
<a href="https://2011.igem.org/Team:NCTU_Formosa/contributions"><img
 +
 
 +
src="https://static.igem.org/mediawiki/2011/4/49/Attribution.jpg" alt=""
 +
 
 +
height="60"/></a>
 +
Attribution
 +
<br><br>
 +
<a href="https://2011.igem.org/Team:NCTU_Formosa/introduction"><img
 +
 
 +
src="https://static.igem.org/mediawiki/2011/e/ea/Project.jpg" alt="" height="60" /></a>
 +
Project
 +
<a href="https://2011.igem.org/Team:NCTU_Formosa/parts"><img
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 +
src="https://static.igem.org/mediawiki/2011/7/7a/Part.jpg" alt="" height="60"/></a>
 +
Parts
 +
<a href="https://2011.igem.org/Team:NCTU_Formosa/modeling"><img
 +
 
 +
src="https://static.igem.org/mediawiki/2011/d/d3/Measurement.jpg" alt=""
 +
 
 +
height="60"/></a>
 +
Measurement
 +
<br><br>
 +
<a href="https://2011.igem.org/Team:NCTU_Formosa/protocol_G"><img
 +
 
 +
src="https://static.igem.org/mediawiki/2011/9/93/Notebook.jpg" alt="" height="60"
 +
 
 +
/></a>
 +
Notebook
 +
<a href="https://2011.igem.org/Team:NCTU_Formosa/safty"><img
 +
 
 +
src="https://static.igem.org/mediawiki/2011/d/d5/Safety.jpg" alt="" height="60" /></a>
 +
Safety</b>
 +
</div></center>
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<br><br>
</div>
</div>
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<br><br><br>
 
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Latest revision as of 14:08, 18 October 2011



Concept

Achievements

Register our team.

Complete the judging form.

Set up a team wiki.

Prepared a poster and a presentation

Submit 46 new BioBrick parts.

Design several new BioBricks, including Carotenoid synthesis Pathway, Violacein biosynthesis pathways, and Butanol synthesis pathway.The Butanol synthesis pathway can produce Biofuel!

Most of our BioBricks work as expected.

Characterize several BioBricks (BBa_K118008, BBa_K274004).

Our favourites are BBa_K539691, BBa_K539151, BBa_K539281, BBa_K539461, and BBa_K539742.

Incorporated Human Practices and modeling into our design.

Designed Pathway commander, a novel concept to to control a pathway to stop at any intermediate state.

Abstract

As one of the main challenges of in vivo experiments is controlling the flux through a synthetic metabolic pathway, it’s design lies in selecting well- matched genetic components that when coupled, can reliably produce the desired behavior. Although model equations can calculate parameter values, a challenge still remains in selecting the bio-bricks that can reliably implement a desired cellular function with quantitative values. In previous studies, synthetic biologists have created numerous synthetic circuits; each generating different protein expression levels in order to test performance in reliability and consistency, but this process is both tedious and time consuming. To overcome this problem, our team designed a novel circuit design: method_ Pathway Commander. By this design method, we construct a single version of a synthetic metabolic pathway circuit that can use culture temperature shifts to control the expression levels of a series of metabolic proteins at the precise times. We have implemented the Pathway_Commander design in (1) Carotenoid synthesis Pathway, (2) Violacein biosynthesis pathways and (3) Butanol synthesis pathway in E. coli. This circuit design utilizes a temperature controlled system that gives precision control over metabolic protein expression which amounts to optimize the synthesis that can maximize synthesis of a given compound or drug.



Human Practice Members Attribution

Project Parts Measurement

Notebook Safety


Retrieved from "http://2011.igem.org/Team:NCTU_Formosa"