Team:Minnesota/leex4462-06292011000000-73

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Silicatein: Digest of pucBB

Project: Surface Display

Date: Wed, 29 Jun 2011 00:00:00 GMT

Author: Sukdong Lee (leex4462)

Access: Public

Revision History:

  • Thu, 25 Aug 2011 14:42:37 GMT (leex4462): entry created in project'Surface Display' by leex4462 (id=3)
  • Tue, 27 Sep 2011 17:13:13 GMT (admin): entry data updated by admin (id=1)

Purpose:

Prepare pucBB plasmid for digestion. Silicatein doesn't need to be digested since it was made with the overhangs.


Procedures:

Same, but digest for 2 hours.


Notes:

-Silicatein= 1kb

-pucBB=2.1 kb

-BglII and NotI are the restriction sites.


Leex4462-06292011000000-731.png

Revision: Updated 9/27/2011 to include image.



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