Team:Minnesota/leex4462-04142011000000-21

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== PCR Amplification of OmpA == Project: Surface Display Date: Thu, 14 Apr 2011 00:00:00 GMT Author: Sukdong Lee (leex4462) Access: Public Revision History:
  • Mon, 22 Aug 2011 09:13:32 GMT (leex4462): entry created in project'Surface Display' by leex4462 (id=3)
Purpose: To replicate the ompA gene and place into a pucBB plasmid. Procedures: Followed standard protocol. For 50 ul reaction add: -1 uL Template -1 uL of each primer -5 uL 10x Thermobuffer -1 uL 10mM ddNTPs -2.5 uL 10mM MgSO4 -0.5 uL DNA polymerase:Vent -38 uL ddH20
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