Team:Minnesota/leex4462-04072011112000-18

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Latest revision as of 22:47, 27 September 2011

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Primer Design for OmpA

Project: Surface Display

Date: Thu, 07 Apr 2011 11:20:00 GMT

Author: Sukdong Lee (leex4462)

Access: Public

Revision History:

Mon, 22 Aug 2011 09:05:03 GMT (leex4462): entry created in project'Surface Display' by leex4462 (id=3)

Purpose:

Designed primers for the E. coli BL21 strain of E. coli to obtain the OmpA gene for the silicatein surface display.

We designed the primers to have the BglII at the beginning. Then the gene followed by a NheI site which ended with a stop codon and a NotI site. The NheI site is so that we can cut the gene out later for the fusion.


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 <nowiki>We designed the primers to have the BglII at the beginning. Then the gene followed by a NheI site which ended with a stop codon and a NotI site. The NheI site is so that we can cut the gene out later for the fusion. </nowiki>
 {{Team:Minnesota/Bottom}}