Team:Minnesota/davis950-08162011135544-17

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<br>== Digest of pcyA and ho1 ==
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== Digest of pcyA and ho1 ==
'''Project:''' Regulatory
'''Project:''' Regulatory
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<nowiki>ho1 digest product should be treated with phosphatase to prevent religation to self. pcyA digest should be gel purified and extracted to remove backbone. These two products should be ligated together, transformed and a colony PCR should verify which colonies contain the full construct.</nowiki>
<nowiki>ho1 digest product should be treated with phosphatase to prevent religation to self. pcyA digest should be gel purified and extracted to remove backbone. These two products should be ligated together, transformed and a colony PCR should verify which colonies contain the full construct.</nowiki>
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{{Team:Minnesota/Bottom}}
{{Team:Minnesota/Bottom}}

Latest revision as of 22:46, 27 September 2011

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Digest of pcyA and ho1

Project: Regulatory

Date: Tue, 16 Aug 2011 13:55:44 GMT

Author: Nathan Davis (davis950)

Access: Public

Revision History:

  • Tue, 16 Aug 2011 13:58:27 GMT (davis950): entry created in project'Regulatory' by davis950 (id=2)

Digest of pcyA and ho1 set up as follows:


  • pcyA digested with XbaI and SpeI
  • ho1 digested with SpeI and PstI


ho1 digest product should be treated with phosphatase to prevent religation to self. pcyA digest should be gel purified and extracted to remove backbone. These two products should be ligated together, transformed and a colony PCR should verify which colonies contain the full construct.


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