Team:MIT

From 2011.igem.org

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<!-- *** What falls between these lines is the Alert Box!  You can remove it from your pages once you have read and understood the alert *** -->
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{{MIT-header}}
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{{MIT-style}}
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<div id="template" style="text-align: center; font-weight: bold; font-size: large; color: #f6f6f6; padding: 5px;">
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This is a template page. READ THESE INSTRUCTIONS.
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2009.igem.org/Help:Template/Examples">HERE</a>.
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You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, a lab notebook, and a safety page.  PLEASE keep all of your pages within your teams namespace. 
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<h3>Tissues by Design</h3>
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<p>Our project focuses on tissue self-construction to achieve specific patterns of cell differentiation (initially with fluorescence, ultimately with cell fate regulators) with genetic circuits. To accomplish this, we focused on three components: cell-cell communication pathways, intracellular information processing circuits, and cell-cell adhesion. Through engineered control of these mechanisms, we are investigating how programmed local rules of interactions between cells can lead to the emergence of desired global patternings.</p>
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<p><img src="https://static.igem.org/mediawiki/2011/5/51/Simulation.jpg" style="max-width:800px; margin-right:10px;"/></p></br>
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<p>Above is the result of a simulation run, starting with undifferentiated cells and ending with a pattern.</p>
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<p>Specifically, for cell-cell signaling, we developed a modular juxtacrine platform, using Notch and Delta proteins. For intracellular information processing circuits, as a proof of concept, we build a 2-input AND gate. For cell-cell adhesion, the final output of our system, we used cadherin.
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|You can write a background of your team here. Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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Below is an animation depicting our project components. The cell-cell signaling of Notch-Delta interaction leads to the cleavage of the Notch intracellular domain, which enters the nucleus and after logic processing, expresses cadherins, which cause cells to adhere to similarly expressing cells.
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|[[Image:MIT_logo.png|200px|right|frame]]
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''Tell us more about your project. Give us background.  Use this as the abstract of your project. Be descriptive but concise (1-2 paragraphs)''
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|[[Image:MIT_team.png|right|frame|Your team picture]]
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|align="center"|[[Team:MIT | Team Example]]
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<!--- The Mission, Experiments --->
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We developed software tools to model the behavior of our system. Below is a sample of a simulation of cells with genetic circuits and how they differentiate.
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<div align="center"><iframe width="400" height="300" src="http://www.youtube.com/embed/dbz4VegsJOw?rel=0&amp;hd=1" frameborder="0" allowfullscreen></iframe>
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    <div id="center">
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!align="center"|[[Team:MIT|Home]]
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        <div id="partners">
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!align="center"|[[Team:MIT/Team|Team]]
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            <h3>Sponsors</h3>
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!align="center"|[https://igem.org/Team.cgi?year=2011&team_name=MIT Official Team Profile]
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    <ul>
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!align="center"|[[Team:MIT/Project|Project]]
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        <li><a href="http://www.bbn.com/"><img src='https://static.igem.org/mediawiki/2011/0/03/Mit-bbn.jpg' /></a></li>
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!align="center"|[[Team:MIT/Parts|Parts Submitted to the Registry]]
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        <li><a href="http://www.geneious.com"><img src='https://static.igem.org/mediawiki/2011/6/65/Mit-geneious.jpg' /></a></li>
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!align="center"|[[Team:MIT/Modeling|Modeling]]
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        <li><a href="http://www.genewiz.com"><img src='https://static.igem.org/mediawiki/2011/3/33/Mit-genewiz.jpg' /></a></li>
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!align="center"|[[Team:MIT/Notebook|Notebook]]
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        <li><a href="http://www.neb.com"><img src='https://static.igem.org/mediawiki/2011/4/4d/Mit-neb.jpg' /></a></li>
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!align="center"|[[Team:MIT/Safety|Safety]]
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        <li><a href="http://www.embitec.com"><img src='https://static.igem.org/mediawiki/2011/7/75/Mit-embitec.jpg' style="width:175px" /></a></li>
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!align="center"|[[Team:MIT/Attributions|Attributions]]
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                <li><a href="http://ebics.net"><img src='https://static.igem.org/mediawiki/igem.org/0/0d/EBICS_logo.JPG' style="width:175px"></a></li>
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                <li><a href="http://ginkgobioworks.com/"><img src='http://ginkgobioworks.com/images/ginkgobioworks_logo.png' style="width:175px; "></a></li>
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                <li><a href="https://2011.igem.org/Main_Page"><img src='https://static.igem.org/mediawiki/igem.org/d/de/IGEM_basic_Logo_stylized.png' style="width:175px;"></li>
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    </ul>
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<div id="gold_sponsors">
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            <h3>Gold Sponsors</h3>
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    <ul>
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    <li><a href="http://www.emdchemicals.com"><img src='https://static.igem.org/mediawiki/2011/e/ee/Gold_emdmillipore.jpg' /></a></li>
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    </ul>
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</div>
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<div id="contributing_departments">
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    <h3>MIT Departments</h3>
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    <ul>
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<li><a href="http://www.csail.mit.edu/"><img src='https://static.igem.org/mediawiki/2011/9/99/Mit-csail.jpg' /></a></li>
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<li><a href="http://www.eecs.mit.edu/"><img src='https://static.igem.org/mediawiki/2011/2/22/Mit-eecs.jpg' /></a></li>
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<li><a href="http://web.mit.edu/be/"><img src='https://static.igem.org/mediawiki/2011/a/a7/Mit-be.jpg' /></a></li>
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                <li><a href="http://web.mit.edu/biology/www/"><img src='https://static.igem.org/mediawiki/2011/b/bf/Mitbiologo.jpg' /></a></li>
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    </ul>
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Latest revision as of 04:03, 29 October 2011

Tissues by Design

Our project focuses on tissue self-construction to achieve specific patterns of cell differentiation (initially with fluorescence, ultimately with cell fate regulators) with genetic circuits. To accomplish this, we focused on three components: cell-cell communication pathways, intracellular information processing circuits, and cell-cell adhesion. Through engineered control of these mechanisms, we are investigating how programmed local rules of interactions between cells can lead to the emergence of desired global patternings.



Above is the result of a simulation run, starting with undifferentiated cells and ending with a pattern.

Specifically, for cell-cell signaling, we developed a modular juxtacrine platform, using Notch and Delta proteins. For intracellular information processing circuits, as a proof of concept, we build a 2-input AND gate. For cell-cell adhesion, the final output of our system, we used cadherin. Below is an animation depicting our project components. The cell-cell signaling of Notch-Delta interaction leads to the cleavage of the Notch intracellular domain, which enters the nucleus and after logic processing, expresses cadherins, which cause cells to adhere to similarly expressing cells.



We developed software tools to model the behavior of our system. Below is a sample of a simulation of cells with genetic circuits and how they differentiate.

Sponsors

MIT Departments

Retrieved from "http://2011.igem.org/Team:MIT"