|
|
| (3 intermediate revisions not shown) |
| Line 1: |
Line 1: |
| - | {{Lyon-INSA-ENS/header}}
| |
| - | {{Lyon-INSA-ENS/blocStyle}}
| |
| - | {{INSA-Lyon/styletestaurelie}}
| |
| - | {{Lyon-INSA-ENS/menuhorizontalProject}}
| |
| - | {{Lyon-INSA-ENS/menuProjectPresentation}}
| |
| | | | |
| - | <html>
| |
| - |
| |
| - |
| |
| - |
| |
| - | <style type="text/css";>
| |
| - | .firstHeading{background-image: url(https://static.igem.org/mediawiki/2011/0/0a/Banniere_centrale_nucleaire.jpg);background-repeat: no repeat;}
| |
| - | </style>
| |
| - |
| |
| - | <div id="language";>
| |
| - | <img src="https://static.igem.org/mediawiki/2011/0/0e/Drapeau_francais.jpg"; width=20px; /> <a href="https://2011.igem.org/Team:Lyon-INSA-ENS/Project/PresentationFr">Version Francaise</a>
| |
| - | </div>
| |
| - |
| |
| - |
| |
| - |
| |
| - | <br><br>
| |
| - | <br><br><br/><br/>
| |
| - |
| |
| - | <!-- Description projet -->
| |
| - |
| |
| - |
| |
| - | <br/>
| |
| - | <p> <font color="green" size="5,5">
| |
| - | A project anchored at the heart of current concerns<br><HR>
| |
| - | </font>
| |
| - | </p>
| |
| - | <br/><br/>
| |
| - |
| |
| - | <p class="contenu">
| |
| - | <span style="line-height : 2em">
| |
| - |
| |
| - | The activity of modern nuclear power plants with pressurized water reactors generates
| |
| - | radioactive effluents that contain among other things radioactive cobalt. The tubing of
| |
| - | the cooling circuit is made of a steel alloy rich in stable cobalt (<sup>59</sup>Co). Undergoing neutron
| |
| - | bombardment coming from the reactor, this stable cobalt changes into its radioactive isotope,
| |
| - | cobalt 60 (<sup>60</sup>Co).
| |
| - |
| |
| - | <br/><br/>
| |
| - |
| |
| - | The capture of this metal is interesting on a sanitary point of view, because it represents
| |
| - | a danger under both its radioactive and stable forms (carcinogenic). It also represents an
| |
| - | advantage on an environmental point of view, in order to avoid contamination of waters, soil
| |
| - | and groundwater. Even with a short half life, cobalt 60 emits high intensity gamma rays, and
| |
| - | decays to nickel, which is stable but polluting.
| |
| - |
| |
| - | <br/><br/>
| |
| - |
| |
| - | Controlled immobilization of radioactive cobalt is both an important sanitary and environmental
| |
| - | issue, which we intend to solve with an innovative and economical response. A researcher
| |
| - | from the Lyon INSA-ENS team, Agnès Rodrigues, has recently constructed a E.coli strain able
| |
| - | to eliminate 85% of radioactive cobalt (<sup>60</sup>Co), initially present as traces in a simulated nuclear
| |
| - | effluent made up of a mix of heavy metals, in only twice one-hour incubation (Appl Microbio
| |
| - | Biotechnol 2009 81:571- 578).
| |
| - |
| |
| - | <br/><br/>
| |
| - |
| |
| - | The process that was developed by Agnès Rodrigue’s team ensures the decontamination
| |
| - | of cobalt up to 0,5 ppm (8 nM in 100 000L) with only 4kg of bacteria as against 50kg with
| |
| - | an unmodified bacterium or 8,000kg of an ion-exchange polymer. This kind of process with
| |
| - | modified bacteria will be a good value because the production of bacteria in a bioreactor is
| |
| - | rather economical. However, one issue remained unsolved at the end of this study, that is the
| |
| - | separation of cobalt-fixing bacteria.
| |
| - |
| |
| - | <br/><br/>
| |
| - |
| |
| - | The first objective of our project is, with the most recent genetic engineering techniques, to
| |
| - | induce the fixation of optimized bacteria for the cobalt capture and retention in response to the
| |
| - | presence of contaminants in the effluent to be treated.
| |
| - |
| |
| - | <br/><br/>
| |
| - |
| |
| - | A second objective aims to develop a system to construct custom-built “biofilm inducible”
| |
| - | strains. Our goal is to construct captors able to launch the formation of biofilm in response to
| |
| - | the presence of various radioactive or not pollutants, and to offer more efficient and cheaper
| |
| - | bioremediation processes.
| |
| - |
| |
| - | <br/><br/>
| |
| - |
| |
| - | To conclude, our objective is to deposit a part able to make any strains inducible to cobalt. In presence of this element, strains will become adherent and will form biofilm thanks to their curli.
| |
| - |
| |
| - | <br/><br/><br/>
| |
| - | </span>
| |
| - | </p>
| |
| - | </html>
| |
| - |
| |
| - | {{Lyon-INSA-ENS/footer}}
| |
"
