Confirmation Gel of I1343, B0034, C0040 & B0015 - Each of I1343, B0034, C0040 & B0015 were restricted following the Restriction Protocol 4.
- A gel was made following the 1% Agarose Gel Protocol (100mL).
- The restrictions were loaded onto the gel with a 1kb ladder and 100bp ladder.
- The gel ran at 120V for 60 minutes.
- The gel was then stained in Ethidium Bromide for 10 minutes.
- Results: The parts were confirmed with the correct sizes.
April 2, 2011
Assembly of I1343 & B0034 and C0040 & B0015 - Each of I1343, B0034, C0040 & B0015 were restricted following the Restriction Protocol 1 where all odd parts are upstream and even parts are downstream.
- I1343 & B0034 were ligated together in pSB1A3 following the Ligation Protocol 1.
- C0040 & B0015 were ligated together in pSB1A3 following the Ligation Protocol 1.
- Both ligations were transformed following the Transformation Protocol 1.
April 21, 2011
April 23, 2011
April 27, 2011
April 28, 2011
May
May 18, 201
Transformation of P0440 in pSB1A2 from 2010 Kit Plate - 10µL of Milli-Q water and extraction of P0440 in pSB1A2 from the 2010 kit plate (10I) were mixed together.
- The transformation was done following the Transformation Protocol 1.
May 20, 2011
Miniprep of P0440 in pSB1A2 Miniprep of P0440 in pSB1A2 was made following the Miniprep Protocol (Modified Bench Protocol: QIAprep Spin Miniprep Kit Using Microcentrifuge).
Assembly of Multiple Parts - Each of I13453, P0440, K331033, K331035, K331035, B0015, K249002, B0015, R0010 & B0034 were restricted following the Restriction Protocol 1 where all odd parts are upstream and even parts are downstream.
- I1343 & P0330 were ligated together in pSB1A3 following the Ligation Protocol 1.
- K331033 & K331035 were ligated together in pSB1A3 following the Ligation Protocol 1.
- K331035 & B0015 were ligated together in pSB1A3 following the Ligation Protocol 1.
- K249002 & B0015 were ligated together in pSB1A3 following the Ligation Protocol 1.
- R0010 & B0034 were ligated together in pSB1A3 following the Ligation Protocol 1.
- All ligations and a negative control (water) were transformed following the Transformation Protocol 1.
- Gel
May 26, 2011
Transformation of K325909 in pSB1C3 and K325229 in pSB1C3 from 2011 Kit Plate - 10µL of Milli-Q water and extraction of K325909 in pSB1C3 from the 2011 kit plate (17m) were mixed together.
- 10µL of Milli-Q water and extraction of K325299 in pSB1C3 from the 2011 kit plate (17c) were mixed together.
- The transformation was done following the Transformation Protocol 1.
June 8, 2011
Minipreps of Multiple Parts - Minipreps of K331022, K331008, K331024, xylE, K331024, K331007, K331022, K331008 & K331007 were done following the Miniprep Protocol (Modified Bench Protocol: QIAprep Spin Miniprep Kit Using Microcentrifuge).
June 9, 2011
Assembly of pBAD-rbs & lumazine-dt - Each of pBAD-rbs & lumazine-dt were restricted following the Restriction Protocol 1
- pBAD-rbs & lumazine-dt were ligated together in pSB1C3 following the Ligation Protocol 1.
- The ligation was transformed following the Transformation Protocol 1.
June 10, 2011
Transformation of pBAD-P0440 - pBAD-P0440 in pSB1K3 was transformed following the Transformation Protocol 1.
Overnight Cultures of pBAD-rbs & lumazine-dt
- Overnight cultures of pBAD-rbs & lumazine-dt grown following the Overnight Cultures Protocol.
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