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Team:Lethbridge/Notebook/Lab Work/Group1
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| + | =April= | ||
| + | ==April 1, 2011== | ||
| + | <br>- Confirmation Gel of I1343, B0034, C0040 & B0015</br> | ||
| + | <br>- Each of I1343, B0034, C0040 & B0015 were restricted following the Restriction Protocol 4.</br> | ||
| + | <br>- A gel was made following the 1% Agarose Gel Protocol (100mL).</br> | ||
| + | <br>- The restrictions were loaded onto the gel with a 1kb ladder and 100bp ladder.</br> | ||
| + | <br>- The gel ran at 120V for 60 minutes.</br> | ||
| + | <br>- The gel was then stained in Ethidium Bromide for 10 minutes.</br> | ||
| + | <br>- Results: The parts were confirmed with the correct sizes.</br> | ||
| + | |||
| + | ==April 2, 2011== | ||
| + | <br>- Assembly of I1343 & B0034 and C0040 & B0015</br> | ||
| + | <br>- Each of I1343, B0034, C0040 & B0015 were restricted following the Restriction Protocol 1 where all odd parts are upstream and even parts are downstream.</br> | ||
| + | <br>- I1343 & B0034 were ligated together in pSB1A3 following the Ligation Protocol 1. </br> | ||
| + | <br>- C0040 & B0015 were ligated together in pSB1A3 following the Ligation Protocol 1. </br> | ||
| + | <br>- Both ligations were transformed following the Transformation Protocol 1.</br> | ||
| + | |||
| + | ==April 21, 2011== | ||
| + | ==April 23, 2011== | ||
| + | ==April 27, 2011 == | ||
| + | ==April 28, 2011== | ||
Revision as of 18:58, 15 September 2011
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