| JM109 | endA, recA1, gyrA96,thi,hsdR17,(rκ-,mκ+),relA1, supE44,
λ-,Δ(lac-proAB),[F',traD36,proAB,laclqZΔM15]
| Promega[http://www.promega.com/products/cloning-and-dna-markers/cloning-tools-and-competent-cells/bacterial-strains-and-competent-cells/bacterial-strain-jm109_de3_/]
|
| Name | Genotype and/or Relevant Features | Source or Reference
|
| TOP10 | F-, mcrA, Δ(mrr-hsdRMS-mcrBC), φ80lacZΔM15, ΔlacX74, nupG, recA1, araD139, Δ(ara-leu)7697, galE15, galK16 rpsL(StrR), endA1, λ- | Invitrogen
|
| KRX | [F′, traD36, ΔompP, proA+B+, lacIq, Δ(lacZ)M15] ΔompT, endA1, recA1, gyrA96 (Nalr), thi-1, hsdR17 (rk–, mk+), e14– (McrA–), relA1, supE44, Δ(lac-proAB), Δ(rhaBAD)::T7 RNA polymerase. | Laboratory of Gene Biodynamics, Graduate School of Biostudies, Kyoto University
|
| (Promega [http://www.promega.com/catalog/catalogproducts.aspx?categoryname=productleaf_1698])
|
| BL21(DE3)pLysS | F–, ompT, hsdSB (rB–, mB–), dcm, gal, λ(DE3), pLysS, Cmr. | Promega [http://www.promega.com/catalog/catalogproducts.aspx?categoryname=productleaf_1005]
|
| Date | Name | Sequence
|
| 07/21 | S Forward (Same as SRRz Forward) | gGAATTCGCGGCCGCTTCTAGAGgcctggatttgttctatcagtaatcga
|
| 07/21 | S Reverse | CTGCAGCGGCCGCTACTAGTAttattgatttctaccatcttctactccggc
|
| 07/21 | SRRz Reverse | CTGCAGCGGCCGCTACTAGTAtgagttgcccatcgatatgggc
|
| 07/28 | ΔTMD1 Forward | cgcggcagatataatggcggt
|
| 07/28 | ΔTMD1 Reverse | gtcatgtttttctggcatcttcatgtct
|
| 08/19, 08/24 | Sam7 Forward | ggttcattcgtgaccttctcgacttc
|
| 08/19, 08/24 | Sam7 Reverse | aggcgataatggcgcacatc
|
| 08/20 | SRRz Forward Retry | gGAATTCGCGGCCGCTTCTAGAGgagcaaatccccttattgggggtaagacatg
|
| 08/20 | SRRz Reverse Retry | CTGCAGCGGCCGCTACTAGTAgcaactctatctgcactgctcattaatatacttctgg
|
| 08/23, 08/24 | Deletion of Upstream Forward | gagcaaatccccttattgggggtaagacatg
|
| 08/23, 08/24 | Deletion of Upstream Reverse | ctctagaagcggccgcgaattcc
|
| 09/13, 09/22 | Add J23105 Forward | CAGTCCTAGGTACTATGCTAGCtactagtagcggccgctgc
|
| 09/13, 09/22 | Add J23105 Reverse | AGCTAGCCGTAAActctagtatataaacgcagaaaggcccacc
|
| 10/10 | Deletion of GFP Forward (Primer1) | ccaggcatcaaataaaacgaaaggctc
|
| 10/10 | Deletion of GFP-DT Forward (Primer2) | tactagtagcggccgctgc
|
| 10/10 | Deletion of GFP, GFP-DT Reverse (Primer3) | ctctagtattattgatttctaccatcttctactcc
|
| - | VF2 (<partinfo>G00100</partinfo>) | tgccacctgacgtctaagaa
|
| - | VF (<partinfo>G00101</partinfo>) | attaccgcctttgagtgagc
|
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