Team:KULeuven/Project

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<h3>E.D. FROSTI: CONTROLLING ICE FORMATION</h3>
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Our team will engineer a bacterium that can perform 2 different functions; depending on the stimulus used, it will either induce ice crystallization or inhibit ice formation.
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    <td align="center" valign="bottom"><img src="http://www.shbts.nl/igem/images/project/icon_inp.png" width="23" height="28" /></td>
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To stimulate ice formation, we will let our bacterium produce Ice Nucleating Proteins (INP), which stimulate the formation of ice crystals. These bacteria could be used in lakes to make the ice stronger, so maybe in future, we can once again have a "11-stedentocht" in the Netherlands. You could use this property as well for the creation of fluorescent ice on ski-slopes or for trendy cocktails with glowing ice cubes in it. Also, it would decrease ice-melting of glaciers, thereby effectively slowing down global warming.
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    <td align="center" valign="bottom"><img src="http://www.shbts.nl/igem/images/project/icon_afp.png" width="23" height="28" /></td>
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The second function of E.D. Frosti is the anti-freeze function by the production of Anti Freeze Proteins (AFP). These bacteria could then be used as anti-freeze biofilms on roads, which would help the roads become ice and snow free in winters. Also other applications which require ice melting could benefit from our bacterium.<br><br><br>
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    <td colspan="3" align="center" valign="middle"><img src="http://www.shbts.nl/igem/images/project/arrows.png" width="257" height="82" style="padding:0 0 20px 0;" /></td>
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    <td align="center" valign="middle"><img src="http://www.shbts.nl/igem/images/project/lactose01_cell.png" alt="" width="130" height="130" /></td>
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    <td align="center" valign="middle"><span class="schematics_txt">Induction of biological properties</span></td>
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    <td align="center" valign="middle"><img src="http://www.shbts.nl/igem/images/project/arabinose01_cell.png" width="130" height="130" /></td>
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    <td align="center" valign="middle"><img src="http://www.shbts.nl/igem/images/project/lactose_arrow_down.png" width="27" height="62" style="padding:40px 0 30px 0;" /></td>
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    <td align="center" valign="middle">&nbsp;</td>
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    <td align="center" valign="middle"><img src="http://www.shbts.nl/igem/images/project/arabinose_arrow_down.png" width="27" height="62" style="padding:40px 0 30px 0;" /></td>
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    <td align="center" valign="middle"><img src="http://www.shbts.nl/igem/images/project/lactose02_death.png" alt="" width="130" height="130" /></td>
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    <td align="center" valign="middle"><span class="schematics_txt">Application at low temperatures</span></td>
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    <td align="center" valign="middle"><img src="http://www.shbts.nl/igem/images/project/arabinose02_death.png" alt="" width="130" height="130" /></td>
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    <td align="center" valign="middle"><img src="http://www.shbts.nl/igem/images/project/logo100x100.png" alt="" width="100" height="100" /></td>
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    <td colspan="4"><p>After stimulation, E.D.Frosti will acquire its desired biological property, i.e. the expression of  INP to induce ice formation, or the expression of a membrane-attached AFP to  stimulate ice melting. Upon application in the environment at low temperatures, a cell death mechanism will kill off the cells, though  the desired biological activity will remain. This mechanism  ensures that the bacteria cannot overgrow in the environment. </p>
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    <p>Importantly, without any  preceding stimulus, no cell death will be induced at low temperatures, so our organism can be safely stored in the fridge.&#13;</p></td>
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<a href="http://www.shbts.nl/igem/images/kugroup_large.jpg" rel="lightbox" title="iGem KULeuven team 2011"><img src="http://www.shbts.nl/igem/images/kugroup.jpg" name="kugroup" width="560" height="255" border="0" id="kugroup" /></a></div><br>
 
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Revision as of 11:51, 3 August 2011

KULeuven iGEM 2011

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Induction of biological properties
 
Application at low temperatures

After stimulation, E.D.Frosti will acquire its desired biological property, i.e. the expression of INP to induce ice formation, or the expression of a membrane-attached AFP to stimulate ice melting. Upon application in the environment at low temperatures, a cell death mechanism will kill off the cells, though  the desired biological activity will remain. This mechanism ensures that the bacteria cannot overgrow in the environment.

Importantly, without any preceding stimulus, no cell death will be induced at low temperatures, so our organism can be safely stored in the fridge.

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