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<div id="contentbox" style="text-align:justify;">
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<h3>E.D. FROSTI Project overview</h3>
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<h3><i>E.D. FROSTI</i> Project overview</h3>
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     <td colspan="4"><br><p>After stimulation, <i>E.D.Frosti</i> will acquire its desired biological property, i.e. the expression of  <b> Ice Nucleation Protein (INP) to induce ice formation</b>, or the expression of a membrane-attached <b> Antifreeze Protein (AFP) to  prevent ice formation</b>. Upon application in the environment at low temperatures, a cell death mechanism will kill off the cells, though the desired biological activity of the proteins on the membranes will remain. This mechanism  ensures that the bacteria cannot overgrow the environment. </p>
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    <td align="center" valign="middle"><img src="http://www.shbts.nl/igem/images/project/lactose01_cell.png" alt="" width="130" height="130" /></td>
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    <td align="center" valign="top"><span class="schematics_txt">Induction of biological properties</span></td>
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    <td align="center" valign="middle">&nbsp;</td>
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    <td align="center" valign="middle"><img src="http://www.shbts.nl/igem/images/project/arabinose_arrow_down.png" width="27" height="62" style="padding:40px 0 30px 0;" /></td>
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    <td align="center" valign="middle"><img src="http://www.shbts.nl/igem/images/project/lactose02_death.png" alt="" width="130" height="130" /></td>
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    <td align="center" valign="top"><span class="schematics_txt">Application at low temperatures</span></td>
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     <td colspan="4"><br><p>After stimulation, <i>E.D.Frosti</i> will acquire its desired biological property, i.e. the expression of  <b> Ice Nucleation Protein (INP) to induce ice formation</b>, or the expression of a membrane-attached <b> Antifreeze Protein (AFP) to  stimulate ice melting</b>. Upon application in the environment at low temperatures, a cell death mechanism will kill off the cells, though  the desired biological activity will remain. This mechanism  ensures that the bacteria cannot overgrow the environment. </p>
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     <p>Importantly, without any  preceding stimulus, no cell death will be induced at low temperatures, so our  organism can be safely stored in the fridge.&#13;</p><br></td>
     <p>Importantly, without any  preceding stimulus, no cell death will be induced at low temperatures, so our  organism can be safely stored in the fridge.&#13;</p><br></td>
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Latest revision as of 12:42, 27 October 2011

KULeuven iGEM 2011

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E.D. FROSTI Project overview


After stimulation, E.D.Frosti will acquire its desired biological property, i.e. the expression of Ice Nucleation Protein (INP) to induce ice formation, or the expression of a membrane-attached Antifreeze Protein (AFP) to prevent ice formation. Upon application in the environment at low temperatures, a cell death mechanism will kill off the cells, though the desired biological activity of the proteins on the membranes will remain. This mechanism ensures that the bacteria cannot overgrow the environment.

Importantly, without any preceding stimulus, no cell death will be induced at low temperatures, so our organism can be safely stored in the fridge.




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