Team:KULeuven/Data

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KULeuven iGEM 2011

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How does E.D. Frosti work?



When Lactose is added to the medium, plasmid 1 will be induced to form INP via BBa_K584028.
When L-arabinose is added to the medium, plasmid 2 will be induced to form AFP via.



Data for our favorite new parts

  • 1. Main page (link) - Constitutive Promoter + INP , BBa_K584027 (KULeuven, iGEM 2011): Tested with MilliQ water (first purified via reversed osmosis over 0,45 µm then filtered through a 0,22µm filter to remove all nucleating agents) at -6°C. Addition -6°C en gewerkt MilliQ (gedenateraliseerd water) gefilterd via reversed osmosis over 0,45 mM filter en nadien 0,20mM om alle nucleatie agents weg te krijgen.

    Data for pre-existing parts

  • 1. Experience (link) - pBAD promoter, BBa_I13453 (Endy Lab, iGEM 2005): Fused a GFP reporter to this promoter (which created biobrick: BBa_K584000). The results can be found at the experience page of that promoter.

  • 2. Experience (link) - constitutive promoter, BBa_J23119 (Berkeley, iGEM 2006): Fused a GFP reporter to this promoter (which created biobrick: BBa_K584001

    We've also characterized the following parts