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| | <h2>Data for our favorite new parts</h2> | | <h2>Data for our favorite new parts</h2> |
| - | <li> 1. <b> Main page</b> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K584028" target="blank"> ( link) </a> - <b> pLac-Lux hybrid promoter + INP </b>, <b> BBa_K584028 </b> (K.U.Leuven, iGEM 2011): Tested with MilliQ water (first purified via reversed osmosis over 0,45 µm then filtered through a 0,22µm filter to remove all nucleating agents) at -6°C. 2 tests were performed: 1mM or 100mM were added to <i>E.D. Frosti</i>'s medium. Water freezes immediately upon addition in both cases. | + | <li> 1. <b> BBa_K584028</b> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K584028" target="blank"> ( Main Page) </a> - <b> pLac-Lux hybrid promoter + INP </b>, <b> BBa_K584028 </b> (K.U.Leuven, iGEM 2011): Tested with MilliQ water (first purified via reversed osmosis over 0,45 µm then filtered through a 0,22µm filter to remove all nucleating agents) at -6°C. 2 tests were performed: 1mM or 100mM were added to <i>E.D. Frosti</i>'s medium. Water freezes immediately upon addition in both cases. |
| | <br><br> | | <br><br> |
| - | <li> 2. <b> Main page</b> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K584027" target="blank"> ( link) </a> - <b> Constitutive Promoter + INP </b>, <b> BBa_K584027 </b> (K.U.Leuven, iGEM 2011): Tested with MilliQ water (first purified via reversed osmosis over 0,45 µm then filtered through a 0,22µm filter to remove all nucleating agents) at -6°C. Water freezes immediately upon addition. | + | <li> 2. <b> BBa_K584027</b> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K584027" target="blank"> ( Main Page) </a> - <b> Constitutive Promoter + INP </b>, <b> BBa_K584027 </b> (K.U.Leuven, iGEM 2011): Tested with MilliQ water (first purified via reversed osmosis over 0,45 µm then filtered through a 0,22µm filter to remove all nucleating agents) at -6°C. Water freezes immediately upon addition. |
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| - | <li> 3. <b> Main page</b> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K584026" target="blank"> ( link) </a> - <b> constitutive promoter + AFP </b>, <b> BBa_K584026 </b> (K.U.Leuven, iGEM 2011): test | + | <li> 3. <b> BBa_K584026</b> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K584026" target="blank"> ( Main Page) </a> - <b> constitutive promoter + AFP </b>, <b> BBa_K584026 </b> (K.U.Leuven, iGEM 2011): test |
| | <br><br> | | <br><br> |
| | <h2> Data for pre-existing parts</h2> | | <h2> Data for pre-existing parts</h2> |
| - | <li> 1. <b> Experience</b> <a href="http://partsregistry.org/Part:BBa_I13453:Experience#User_Reviews" target="blank"> ( link) </a> - <b> pBAD promoter</b>, <b> BBa_I13453 </b> (Endy Lab, iGEM 2005): Fused a GFP reporter to this promoter (which created biobrick: <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K584000" target="blank"> <b>BBa_K584000</b></a>). The results can be found at the experience page of that promoter.<br><br> | + | <li> 1. <b> BBa_I13453</b> <a href="http://partsregistry.org/Part:BBa_I13453:Experience#User_Reviews" target="blank"> ( Experience) </a> - <b> pBAD promoter</b>, <b> BBa_I13453 </b> (Endy Lab, iGEM 2005): Fused a GFP reporter to this promoter (which created biobrick: <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K584000" target="blank"> <b>BBa_K584000</b></a>). The results can be found at the experience page of that promoter.<br><br> |
| - | <li> 2. <b> Experience</b> <a href="http://partsregistry.org/Part:BBa_J23119:Experience" target="blank"> ( link) </a> - <b> constitutive promoter</b>, <b> BBa_J23119 </b> (Berkeley, iGEM 2006): Fused a GFP reporter to this promoter (which created biobrick: <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K584001" target="blank"> BBa_K584001</a><br><br> | + | <li> 2. <b> BBa_J23119</b> <a href="http://partsregistry.org/Part:BBa_J23119:Experience" target="blank"> ( Experience) </a> - <b> constitutive promoter</b>, <b> BBa_J23119 </b> (Berkeley, iGEM 2006): Fused a GFP reporter to this promoter (which created biobrick: <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K584001" target="blank"> BBa_K584001</a><br><br> |
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| | <h2>We've also characterized the following parts</h2> | | <h2>We've also characterized the following parts</h2> |
| - | <li> 1. <b> Main page</b> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K584004" target="blank"> ( link) </a> - <b> HybB promoter + GFP generator</b>, <b> BBa_K584004 </b> (KULeuven, iGEM 2011): We created this biobrick but due to lack of time we could not test it. | + | <li> 1. <b> BBa_K584004</b> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K584004" target="blank"> ( Main Page) </a> - <b> HybB promoter + GFP generator</b>, <b> BBa_K584004 </b> (KULeuven, iGEM 2011): We created this biobrick but due to lack of time we could not test it. |
| | <br><br> | | <br><br> |
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Data page
How does E.D. Frosti work?
When Lactose is added to the medium, plasmid 1 will be induced to form INP via BBa_K584028.
When L-arabinose is added to the medium, plasmid 2 will be induced to form AFP via.
Data for our favorite new parts
1. BBa_K584028 (Main Page) - pLac-Lux hybrid promoter + INP , BBa_K584028 (K.U.Leuven, iGEM 2011): Tested with MilliQ water (first purified via reversed osmosis over 0,45 µm then filtered through a 0,22µm filter to remove all nucleating agents) at -6°C. 2 tests were performed: 1mM or 100mM were added to E.D. Frosti's medium. Water freezes immediately upon addition in both cases.
2. BBa_K584027 (Main Page) - Constitutive Promoter + INP , BBa_K584027 (K.U.Leuven, iGEM 2011): Tested with MilliQ water (first purified via reversed osmosis over 0,45 µm then filtered through a 0,22µm filter to remove all nucleating agents) at -6°C. Water freezes immediately upon addition.
3. BBa_K584026 (Main Page) - constitutive promoter + AFP , BBa_K584026 (K.U.Leuven, iGEM 2011): test
Data for pre-existing parts
1. BBa_I13453 (Experience) - pBAD promoter, BBa_I13453 (Endy Lab, iGEM 2005): Fused a GFP reporter to this promoter (which created biobrick: BBa_K584000). The results can be found at the experience page of that promoter.
2. BBa_J23119 (Experience) - constitutive promoter, BBa_J23119 (Berkeley, iGEM 2006): Fused a GFP reporter to this promoter (which created biobrick: BBa_K584001
We've also characterized the following parts
1. BBa_K584004 (Main Page) - HybB promoter + GFP generator, BBa_K584004 (KULeuven, iGEM 2011): We created this biobrick but due to lack of time we could not test it.
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