Team:KULeuven/Biobricks

From 2011.igem.org

(Difference between revisions)

Latest revision as of 12:47, 27 October 2011

KULeuven iGEM 2011

Biobricks

The list of all the biobricks that we used are on the iGEM registry which can be found here.

Below you can see two short videos in which we added different kinds of E.D Frosti to supercooled (-6°C) ultrapure “milli-Q” water (Millipore Corporation). The first video (GFP-control), is a control: when E.D. Frosti constitutively expressing a GFP construct is added to the supercooled water, it does not freeze. In the second video (INP), E.D. Frosti contains a constitutively active Ice-Nucleating Protein producing biobrick ( BBa_K584027). When we added this to the supercooled water, it froze immediately.

@@ Line 69: / Line 69: @@
 	</ul></li>
-	<li class="on"><a href="https://2011.igem.org/Team:KULeuven/Project">Project</a>
+	<li class="off"><a href="https://2011.igem.org/Team:KULeuven/Description">Project</a>
 	<ul>
 		<li><a href="#"></a></li>
-		<li><a href="https://2011.igem.org/Team:KULeuven/Project">Summary</a></li>
+		<li><a href="https://2011.igem.org/Team:KULeuven/Description">Description</a></li>
-		<li><a href="https://2011.igem.org/Team:KULeuven/Details">Extended</a><li>
 		<li><a href="https://2011.igem.org/Team:KULeuven/Modeling">Modeling</a></li>
+                <li><a href="https://2011.igem.org/Team:KULeuven/Thermodynamics">Thermodynamics</a></li>
+                <li><a href="https://2011.igem.org/Team:KULeuven/Applications">Applications</a></li>
 		<li><a href="https://2011.igem.org/Team:KULeuven/Biobricks" style="border-bottom:2px solid #000; color:#000;">Biobricks</a></li>
 		<li><a href="https://2011.igem.org/Team:KULeuven/Notebook">Notebook</a></li>
+                <li><a href="https://2011.igem.org/Team:KULeuven/Results">Results</a></li>
 	</ul></li>
@@ Line 97: / Line 99: @@
 		<li><a href="https://2011.igem.org/Team:KULeuven/Ethics">Ethics</a></li>
 		<li><a href="https://2011.igem.org/Team:KULeuven/Safety">Safety</a></li>
+                <li><a href="https://2011.igem.org/Team:KULeuven/Law&Patents">Law&Patents</a></li>
 	</ul></li>
@@ Line 119: / Line 122: @@
 <br>
-The biobricks that we used are on the iGEM registry which can be found<a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&group=KULeuven" target="blank"> here</a>.
+The list of all the biobricks that we used are on the iGEM registry which can be found<a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&group=KULeuven" target="blank"> here</a>. <br><br>
-Underneath you can see several short videos in which we added different kinds of <i>E.D Frosti</i> to supercooled (-6°C)
+Below you can see two short videos in which we added different kinds of <i>E.D Frosti</i> to supercooled (-6°C)
-ultrapure “milli-Q” water (Millipore Corporation). The first video (GFP-control), is a control: <i>E.D. Frosti</i> only contains a GFP construct. When we add it to the supercooled water, it does not freeze.
+ultrapure “milli-Q” water (Millipore Corporation). The first video (GFP-control), is a control: when <i>E.D. Frosti</i> constitutively expressing a GFP construct is added to the supercooled water, it does not freeze.
-<br><br>
+In the second video (INP), <i> E.D. Frosti</i> contains a constitutively active Ice-Nucleating Protein producing biobrick (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K584027" target="blank">  <b>BBa_K584027</b></a>).
-In the second video (IPTG), <i>E.D. Frosti</i> contains <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K584028" target="blank"> <b>BBa_K584028</b> </a>
-which contains the <i>INP</i> gene, coupled to the lactose inducible promoter. We added 1mM IPTG to <i>E.D. Frosti</i>. When we added it to supercooled water, it froze immediately,
-prooving the functionallity of our biobrick. <br><br>
-In the third video (INP), <i> E.D. Frosti</i> contains a constitutively active INP producing biobrick (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K584027" target="blank">  <b>BBa_K584027</b></a>).
 When we added this to the supercooled water, it froze immediately.
 <br><br>
 <center>
-<iframe src="http://player.vimeo.com/video/29380483?title=0&amp;byline=0&amp;portrait=0" width="560" height="315" frameborder="0" webkitAllowFullScreen allowFullScreen></iframe><br><br>
-</center>
+<iframe src="http://player.vimeo.com/video/29380418?title=0&amp;byline=0&amp;portrait=0" width="590" height="315" frameborder="0" webkitAllowFullScreen allowFullScreen></iframe><br><br>
-The fourth video (No IPTG) shows what happened when we added <i>E.D. Frosti </i> with the <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K584028" target="blank"> <b>BBa_K584028</b> </a>
-biobrick, but that did not receive a lactose (IPTG) stimulus. Although our GFP-characterization of this biobrick showed that the promoter was not leaky, the water still froze.
-This can have several causes, two examples are: the coupling of the <i>INP</i> gene to the promoter made the system leaky. It is also possible that there is a very low (basal) expression of the <i>INP</i> gene and that this small amount of INP is enough to induce ice nucleation.
 <br><br>
-<center>
+<iframe src="http://player.vimeo.com/video/29380483?title=0&amp;byline=0&amp;portrait=0" width="590" height="315" frameborder="0" webkitAllowFullScreen allowFullScreen></iframe><br><br>
-<object width="560" height="315"><param name="wmode" value="transparent"><param name="movie" value="http://www.youtube.com/v/2PbQEq0V8yk?version=3&amp;hl=nl_NL&amp;rel=0"></param><param name="allowFullScreen" value="true"></param><param name="allowscriptaccess" value="always"></param><embed src="http://www.youtube.com/v/2PbQEq0V8yk?version=3&amp;hl=nl_NL&amp;rel=0;wmode=transparent" type="application/x-shockwave-flash" width="560" height="315" allowscriptaccess="always" allowfullscreen="true"></embed></object><br><br>
 </center>