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Team:KULeuven/Biobricks
From 2011.igem.org
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The biobricks that we used are on the iGEM registry which can be found<a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&group=KULeuven" target="blank"> here</a>. | The biobricks that we used are on the iGEM registry which can be found<a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&group=KULeuven" target="blank"> here</a>. | ||
Underneath you can see several short videos in which we added different kinds of <i>E.D Frosti</i> to supercooled (-6°C) | Underneath you can see several short videos in which we added different kinds of <i>E.D Frosti</i> to supercooled (-6°C) | ||
| - | ultrapure “milli-Q” water (Millipore Corporation). The first video (GFP-control), is a control: <i>E.D. Frosti</i> | + | ultrapure “milli-Q” water (Millipore Corporation). The first video (GFP-control), is a control: when <i>E.D. Frosti</i> constitutively expressing a GFP construct is added to the supercooled water, it does not freeze. |
<br><br> | <br><br> | ||
| - | + | In the second video (INP), <i> E.D. Frosti</i> contains a constitutively active Ice-Nucleating Protein producing biobrick (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K584027" target="blank"> <b>BBa_K584027</b></a>). | |
When we added this to the supercooled water, it froze immediately. | When we added this to the supercooled water, it froze immediately. | ||
<br><br> | <br><br> | ||
Revision as of 21:40, 21 September 2011
Biobricks
The biobricks that we used are on the iGEM registry which can be found here. Underneath you can see several short videos in which we added different kinds of E.D Frosti to supercooled (-6°C) ultrapure “milli-Q” water (Millipore Corporation). The first video (GFP-control), is a control: when E.D. Frosti constitutively expressing a GFP construct is added to the supercooled water, it does not freeze.
In the second video (INP), E.D. Frosti contains a constitutively active Ice-Nucleating Protein producing biobrick ( BBa_K584027). When we added this to the supercooled water, it froze immediately.
