Team:KIT-Kyoto/ぷろとこる英語

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Revision as of 13:01, 2 October 2011 by Adachi (Talk | contribs)

いんぐりっしゅ!


Ligation

Refer to following table, prepare a reaction solution.

insert0.5 µl
vector0.5 µl
10 x Buffer2.5 µl
T4 ligase0.5 µl
H2O1.0 µl
 total 5 µl


Incubate it for 30min at 16 ℃.