Team:KIT-Kyoto/ぷろとこる英語

From 2011.igem.org

(Difference between revisions)
Line 3: Line 3:
-
Ligation<BR>
+
<p><strong>Ligation</strong>
 +
<BR>
<BR>
<BR>
Refer to following table, prepare a reaction solution.<BR>
Refer to following table, prepare a reaction solution.<BR>
Line 10: Line 11:
<tr><td width="130px" align=center>insert</td><td width="70px" align=right>0.5 µl</td></tr>
<tr><td width="130px" align=center>insert</td><td width="70px" align=right>0.5 µl</td></tr>
<tr><td align=center>vector</td><td align=right>0.5 µl</td></tr>
<tr><td align=center>vector</td><td align=right>0.5 µl</td></tr>
-
<tr><td align=center>10 x Buffer</td><td align=right>2.5 µl</td></tr>
+
<tr><td align=center>2 x Buffer</td><td align=right>2.5 µl</td></tr>
<tr><td align=center>T4 ligase</td><td align=right>0.5 µl</td></tr>
<tr><td align=center>T4 ligase</td><td align=right>0.5 µl</td></tr>
<tr><td align=center>H<sub>2</sub>O</td><td align=right>1.0 µl</td></tr>
<tr><td align=center>H<sub>2</sub>O</td><td align=right>1.0 µl</td></tr>

Revision as of 13:17, 2 October 2011

いんぐりっしゅ!


Ligation

Refer to following table, prepare a reaction solution.

insert0.5 µl
vector0.5 µl
2 x Buffer2.5 µl
T4 ligase0.5 µl
H2O1.0 µl
 total 5 µl


Incubate it for 30min at 16 ℃.