Team:Imperial College London/test9
From 2011.igem.org
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- | + | <a href="#" id="CPEC" rel="subcontent">CPEC</a> | |
- | + | <div id="subcontent" style="position:fixed; visibility: hidden; border: 9px solid #225323; background-color: white; width: 400px; padding: 8px;"> | |
- | < | + | <p>CPEC (Circular Polymerase Extension Cloning) is a primer-independent PCR assembly technique which relies on overlaping sequences between each part to be assembled. With a denaturing step, the double stranded DNA is melted, allowing compatible single stranded ends of each part to joined. For this reason it is essential that the parts are designed with homologous ends (the fragments we used were designed with 60 bp overlaps). The annealed overlapping ends then serve as primers for polymerase extension to join the parts into a seamless construct.</p> |
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//Call dropdowncontent.init("anchorID", "positionString", glideduration, "revealBehavior") at the end of the page: | //Call dropdowncontent.init("anchorID", "positionString", glideduration, "revealBehavior") at the end of the page: | ||
- | + | dropdowncontent.init("CPEC", "right-bottom", 500, "mouseover") | |
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</html> | </html> |
Revision as of 15:40, 21 September 2011
CPEC
CPEC (Circular Polymerase Extension Cloning) is a primer-independent PCR assembly technique which relies on overlaping sequences between each part to be assembled. With a denaturing step, the double stranded DNA is melted, allowing compatible single stranded ends of each part to joined. For this reason it is essential that the parts are designed with homologous ends (the fragments we used were designed with 60 bp overlaps). The annealed overlapping ends then serve as primers for polymerase extension to join the parts into a seamless construct.