Team:Imperial College London/Project/Auxin/Protocols

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<h2>27th of July</h2>
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The strain of E. coli with the copy of super-folded GFP that was incubated the previous night has shown limited/no growth in the prepared LB broth. This could have been caused by:<br>
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-No innoculation<br>
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-Kanamycin concentration in LB broth was too high. We had used 85μg/ml.<br><br>
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<b>New protocol:</b><br>
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50μg/ml is the recommended concentration. We will use 35-40μg/ml of kanamycin to speed up growth.</html>
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Latest revision as of 14:26, 2 August 2011

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